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Sample GSM563211 Query DataSets for GSM563211
Status Public on Jul 21, 2010
Title Human chronic myeloid leukemic cell line LAMA-84.2
Sample type RNA
 
Channel 1
Source name LAMA-84, MSI2 shRNA
Organism Homo sapiens
Characteristics cell line: LAMA-84
treatment: MSI2 shRNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Quick Amp Agilent protocol
 
Channel 2
Source name LAMA-84, control
Organism Homo sapiens
Characteristics treatment: Control
cell line: LAMA-84
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Quick Amp Agilent protocol
 
 
Hybridization protocol Agilent protocol
Scan protocol Agilent protocol
Description Scramble Control shRNA (Cy5) vs msi2 shRNA hairpin2 (Cy3), LAMA-84, dye-swap
Data processing Image intensity data were recorded and transferred into text files using Feature Extraction software (version 8.5). Limma R/BioC package was used for background correction. Loess normalization was applied within arrays and between arrays using a quantile normalization procedure, and log transformed
 
Submission date Jul 06, 2010
Last update date Jul 21, 2010
Contact name Fatima Al-Shahrour
E-mail(s) shahrour@broadinstitute.org
Organization name Broad Institute of MIT and Harvard
Department Cancer program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL4133
Series (2)
GSE22775 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines)
GSE22778 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis

Data table header descriptions
ID_REF
VALUE lowess normalized

Data table
ID_REF VALUE
1 0.145415984
2 0.073889148
3 0.240799775
4 -0.102215585
5 0.269582196
6 0.360403134
7 -0.012452317
8 -0.34199384
9 -0.16310695
10 -0.082353426
11 0.676200619
12 0.074003722
13 -0.515236469
14 -0.75061908
15 -0.444477637
16 0.506226849
17 0.322129626
18 0.208746258
19 0.126078859
20 0.311200932

Total number of rows: 45015

Table truncated, full table size 793 Kbytes.




Supplementary file Size Download File type/resource
GSM563211_251485042608_200907021010_S01_GE2-v5_10_Apr08_1_3.txt.gz 15.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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