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Sample GSM563213 Query DataSets for GSM563213
Status Public on Jul 21, 2010
Title Human chronic myeloid leukemic cell line ARA-230.2
Sample type RNA
 
Channel 1
Source name ARA-230, MSI2 shRNA
Organism Homo sapiens
Characteristics cell line: ARA-230
treatment: MSI2 shRNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Quick Amp Agilent protocol
 
Channel 2
Source name ARA-230, control
Organism Homo sapiens
Characteristics treatment: Control
cell line: ARA-230
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Quick Amp Agilent protocol
 
 
Hybridization protocol Agilent protocol
Scan protocol Agilent protocol
Description Scramble Control shRNA (Cy5) vs msi2 shRNA hairpin2 (Cy3), ARA-230, dye-swap
Data processing Image intensity data were recorded and transferred into text files using Feature Extraction software (version 8.5). Limma R/BioC package was used for background correction. Loess normalization was applied within arrays and between arrays using a quantile normalization procedure, and log transformed
 
Submission date Jul 06, 2010
Last update date Jul 21, 2010
Contact name Fatima Al-Shahrour
E-mail(s) shahrour@broadinstitute.org
Organization name Broad Institute of MIT and Harvard
Department Cancer program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL4133
Series (2)
GSE22775 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines)
GSE22778 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis

Data table header descriptions
ID_REF
VALUE lowess normalized

Data table
ID_REF VALUE
1 -0.132467855
2 -0.286930322
3 -0.606944387
4 -0.3165904
5 -0.283024324
6 -0.368507886
7 -0.491181288
8 -0.324340135
9 0.019444077
10 -0.775470293
11 -0.014222488
12 -0.200810632
13 -0.463611477
14 -0.575121408
15 -0.736037909
16 0.582135561
17 0.096826122
18 -0.299809154
19 0.28653685
20 0.107413178

Total number of rows: 45015

Table truncated, full table size 794 Kbytes.




Supplementary file Size Download File type/resource
GSM563213_251485042608_200907021010_S01_GE2-v5_10_Apr08_1_1.txt.gz 15.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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