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Sample GSM563212 Query DataSets for GSM563212
Status Public on Jul 21, 2010
Title Human chronic myeloid leukemic cell line ARA-230.1
Sample type RNA
 
Channel 1
Source name ARA-230, control
Organism Homo sapiens
Characteristics cell line: ARA-230
treatment: Control
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Quick Amp Agilent protocol
 
Channel 2
Source name ARA-230, MSI2 shRNA
Organism Homo sapiens
Characteristics treatment: MSI2 shRNA
cell line: ARA-230
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Quick Amp Agilent protocol
 
 
Hybridization protocol Agilent protocol
Scan protocol Agilent protocol
Description Scramble Control shRNA (Cy3) vs msi2 shRNA hairpin1 (Cy5), ARA-230
Data processing Image intensity data were recorded and transferred into text files using Feature Extraction software (version 8.5). Limma R/BioC package was used for background correction. Loess normalization was applied within arrays and between arrays using a quantile normalization procedure, and log transformed
 
Submission date Jul 06, 2010
Last update date Jul 21, 2010
Contact name Fatima Al-Shahrour
E-mail(s) shahrour@broadinstitute.org
Organization name Broad Institute of MIT and Harvard
Department Cancer program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL4133
Series (2)
GSE22775 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines)
GSE22778 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis

Data table header descriptions
ID_REF
VALUE lowess normalized

Data table
ID_REF VALUE
1 -0.039982852
2 -0.269432219
3 -0.309560366
4 -0.600023274
5 -0.739333075
6 -0.672281845
7 -0.394227632
8 -0.147142632
9 -0.359439675
10 0.10697626
11 -0.586512979
12 -0.465749612
13 -0.366390851
14 0.321826267
15 0.197700156
16 0.137606314
17 0.016014369
18 -0.412963561
19 0.529276454
20 0.02211715

Total number of rows: 45015

Table truncated, full table size 797 Kbytes.




Supplementary file Size Download File type/resource
GSM563212_251485042608_200907021010_S01_GE2-v5_10_Apr08_1_2.txt.gz 15.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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