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Sample GSM563216 Query DataSets for GSM563216
Status Public on Jul 21, 2010
Title Human acute myeloid leukaemia cell line THP-I.1
Sample type RNA
 
Channel 1
Source name THP-I, control
Organism Homo sapiens
Characteristics cell line: THP-I
treatment: Control
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Quick Amp Agilent protocol
 
Channel 2
Source name THP-I, MSI2 shRNA
Organism Homo sapiens
Characteristics treatment: MSI2 shRNA
cell line: THP-I
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Quick Amp Agilent protocol
 
 
Hybridization protocol Agilent protocol
Scan protocol Agilent protocol
Description Scramble Control shRNA (Cy3) vs msi2 shRNA hairpin1 (Cy5), THP-I
Data processing Image intensity data were recorded and transferred into text files using Feature Extraction software (version 8.5). Limma R/BioC package was used for background correction. Loess normalization was applied within arrays and between arrays using a quantile normalization procedure, and log transformed
 
Submission date Jul 06, 2010
Last update date Jul 21, 2010
Contact name Fatima Al-Shahrour
E-mail(s) shahrour@broadinstitute.org
Organization name Broad Institute of MIT and Harvard
Department Cancer program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL4133
Series (2)
GSE22775 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines)
GSE22778 Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis

Data table header descriptions
ID_REF
VALUE lowess normalized

Data table
ID_REF VALUE
1 -0.274874787
2 -0.560047414
3 -0.15479486
4 -0.600022258
5 -0.488847462
6 -0.345610953
7 -0.560390894
8 -0.644871285
9 -0.223064536
10 -0.525779589
11 -0.302849886
12 -0.680843239
13 -0.284665363
14 0.540220032
15 -0.01337351
16 0.626427252
17 -0.026274347
18 -0.748423153
19 0.641577508
20 0.014720923

Total number of rows: 45015

Table truncated, full table size 799 Kbytes.




Supplementary file Size Download File type/resource
GSM563216_251485042609_200907021136_S01_GE2-v5_10_Apr08_1_2.txt.gz 15.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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