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Series GSE18438 Query DataSets for GSE18438
Status Public on Dec 05, 2015
Title Over-expression of hsa-miR-221 in JSC1 cells and identification of miRNA targets expressed in a panel of B-cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary 1) To identify changes in gene expression upon over-expression of hsa-miR-221 in JSC1 cells. 2) To identify human miRNA targets expressed in a panel of B-cell lines.
Gammaherpesvirus and host cell microRNAs (miRNAs) together modulate gene expression in normal and malignant cells. Using microRNA microarrays, we determined the expression of mature viral and host cellular miRNAs in a series of B cell tumours that include Kaposi’s Sarcoma-associated herpesvirus (KSHV) infected Primary Effusion Lymphoma (PEL) and Epstein-Barr virus (EBV) infected Burkitt’s lymphoma (BL) cell lines. We show that 35 host miRNAs were constitutively expressed in all the B cell lymphomas and differences in viral miRNA expression were evident between herpesvirus positive tumour types. Furthermore, we show that in PEL, miR-221 and miR-222 expression is defective due to a lack of transcript expression rather than mutation in the miRNA encoding loci. Absence of miR-221 and miR-222 resulted in the enhanced expression of the known target gene p27 (CDKN1B) and reintroduction of miR221 in PEL reduces p27 protein expression.
Overall design The dataset comes in two parts. 1) 6 JSC1 samples: 2 with miR-221 lentiviral vector, 2 without and 2 with K5 short hairpin RNA as control. 2) A panel of 14 B-cell line samples. Each of the 20 samples was Cy5 labelled and run with Cy3-labelled Stratagene reference RNA.
Contributor(s) Coulter E, Frampton D, Buck A, Kellam P
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Submission date Oct 06, 2009
Last update date Feb 22, 2018
Contact name Dan Frampton
Organization name UCL
Department Infection
Lab Kellam
Street address Gower Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (20)
GSM459474 JSC1_rep1
GSM459475 JSC1_rep2
GSM459476 JSC1_mir221_rep1
This SubSeries is part of SuperSeries:
GSE18439 Transcription repression of miR-221 leads to p27 expression in PEL
BioProject PRJNA123261

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18438_RAW.tar 293.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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