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Sample GSM459474 Query DataSets for GSM459474
Status Public on Dec 05, 2015
Title JSC1_rep1
Sample type RNA
 
Channel 1
Source name JSC1
Organism Homo sapiens
Characteristics cell line: JSC1
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy5
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
Channel 2
Source name Stratagene reference RNA
Organism Homo sapiens
Characteristics sample: reference RNA
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy3
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
 
Hybridization protocol Hybridization was performed for 17 hrs at 65°C
Scan protocol Arrays were scanned on an Agilent DNA microarray scanner. Images were analyzed and data were extracted using the standard procedures of Agilent Feature Extraction software (v9.5.3.1).
Description Sample used in miR221 experiment
Data processing Scale normalisation across arrays (R/BioConductor), with samples 1-6 and 7-20 being normalised separately.
 
Submission date Oct 06, 2009
Last update date Dec 05, 2015
Contact name Dan Frampton
Organization name UCL
Department Infection
Lab Kellam
Street address Gower Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
 
Platform ID GPL4133
Series (2)
GSE18438 Over-expression of hsa-miR-221 in JSC1 cells and identification of miRNA targets expressed in a panel of B-cell lines
GSE18439 Transcription repression of miR-221 leads to p27 expression in PEL

Data table header descriptions
ID_REF
VALUE Scale normalised log2 ratio (sample/reference)

Data table
ID_REF VALUE
1 0.356459122813368
2 0.108283808730878
3 0.107311754231477
4 0.106528257834746
5 0.105915836614017
6 0.105474317029146
7 0.105191060636664
8 0.105052033943753
9 0.105071437823216
10 0.105235958115478
11 0.105549034910398
12 1.70874174377417
13 2.50990383229682
14 0.94503126104924
15 -1.04899999931244
16 1.05758024671303
17 0.589228211469757
18 -0.618047803178709
19 1.01780346283222
20 2.53452859009279

Total number of rows: 45015

Table truncated, full table size 1043 Kbytes.




Supplementary file Size Download File type/resource
GSM459474_US45102835_251485046356_S01_GE2-v5_95_Feb07_1_4.txt.gz 14.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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