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Sample GSM459482 Query DataSets for GSM459482
Status Public on Dec 05, 2015
Title AKATA_Bcell_panel_rep1
Sample type RNA
 
Channel 1
Source name AKATA_Bcell_panel
Organism Homo sapiens
Characteristics cell line: AKATA
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy5
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
Channel 2
Source name Stratagene reference RNA
Organism Homo sapiens
Characteristics sample: reference RNA
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy3
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
 
Hybridization protocol Hybridization was performed for 17 hrs at 65°C
Scan protocol Arrays were scanned on an Agilent DNA microarray scanner. Images were analyzed and data were extracted using the standard procedures of Agilent Feature Extraction software (v9.5.3.1).
Description Sample used in panel of B-cell lines
Data processing Scale normalisation across arrays (R/BioConductor), with samples 1-6 and 7-20 being normalised separately.
 
Submission date Oct 06, 2009
Last update date Dec 05, 2015
Contact name Dan Frampton
Organization name UCL
Department Infection
Lab Kellam
Street address Gower Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
 
Platform ID GPL4133
Series (2)
GSE18438 Over-expression of hsa-miR-221 in JSC1 cells and identification of miRNA targets expressed in a panel of B-cell lines
GSE18439 Transcription repression of miR-221 leads to p27 expression in PEL

Data table header descriptions
ID_REF
VALUE Scale normalised log2 ratio (sample/reference)

Data table
ID_REF VALUE
1 -1.88959645071449
2 -0.163613530258523
3 -0.160285457945523
4 -0.157083680562882
5 -0.154059936585968
6 -0.151466378183345
7 -0.148825939223233
8 -0.146471374692741
9 -0.144137692966555
10 -0.142081877556032
11 -0.140151326189160
12 -3.67721215276129
13 2.08138117435414
14 0.317740255178645
15 1.56228055294106
16 -0.383321356788429
17 2.24693946128246
18 0.147920610318044
19 0.309643396758006
20 1.64080571466724

Total number of rows: 45015

Table truncated, full table size 1045 Kbytes.




Supplementary file Size Download File type/resource
GSM459482_AKATA.txt.gz 14.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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