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Sample GSM459484 Query DataSets for GSM459484
Status Public on Dec 05, 2015
Title DEL_Bcell_panel_rep1
Sample type RNA
 
Channel 1
Source name DEL_Bcell_panel
Organism Homo sapiens
Characteristics cell line: DEL
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy5
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
Channel 2
Source name Stratagene reference RNA
Organism Homo sapiens
Characteristics sample: reference RNA
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy3
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
 
Hybridization protocol Hybridization was performed for 17 hrs at 65°C
Scan protocol Arrays were scanned on an Agilent DNA microarray scanner. Images were analyzed and data were extracted using the standard procedures of Agilent Feature Extraction software (v9.5.3.1).
Description Sample used in panel of B-cell lines
Data processing Scale normalisation across arrays (R/BioConductor), with samples 1-6 and 7-20 being normalised separately.
 
Submission date Oct 06, 2009
Last update date Dec 05, 2015
Contact name Dan Frampton
Organization name UCL
Department Infection
Lab Kellam
Street address Gower Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
 
Platform ID GPL4133
Series (2)
GSE18438 Over-expression of hsa-miR-221 in JSC1 cells and identification of miRNA targets expressed in a panel of B-cell lines
GSE18439 Transcription repression of miR-221 leads to p27 expression in PEL

Data table header descriptions
ID_REF
VALUE Scale normalised log2 ratio (sample/reference)

Data table
ID_REF VALUE
1 -2.36753340269543
2 -1.29434678203269
3 -1.77460596513360
4 -1.39317685320147
5 -1.69193496717371
6 -0.879510370030496
7 0.218100655791981
8 -0.258144199741474
9 -0.258159865541911
10 -0.258178164168475
11 -0.258147280632187
12 -1.85440256532447
13 2.84445054861511
14 -0.0228604903151886
15 3.12662871520498
16 0.795318277208448
17 -2.21061625229700
18 1.63206048309011
19 0.00577673336031297
20 -1.11748433300537

Total number of rows: 45015

Table truncated, full table size 1045 Kbytes.




Supplementary file Size Download File type/resource
GSM459484_DEL.txt.gz 14.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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