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Sample GSM459476 Query DataSets for GSM459476
Status Public on Dec 05, 2015
Title JSC1_mir221_rep1
Sample type RNA
 
Channel 1
Source name JSC1_mir221
Organism Homo sapiens
Characteristics cell line: JSC1
hairpin: hsa-miR-221
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy5
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
Channel 2
Source name Stratagene reference RNA
Organism Homo sapiens
Characteristics sample: reference RNA
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy3
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
 
Hybridization protocol Hybridization was performed for 17 hrs at 65°C
Scan protocol Arrays were scanned on an Agilent DNA microarray scanner. Images were analyzed and data were extracted using the standard procedures of Agilent Feature Extraction software (v9.5.3.1).
Description Sample used in miR221 experiment
Data processing Scale normalisation across arrays (R/BioConductor), with samples 1-6 and 7-20 being normalised separately.
 
Submission date Oct 06, 2009
Last update date Dec 05, 2015
Contact name Dan Frampton
Organization name UCL
Department Infection
Lab Kellam
Street address Gower Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
 
Platform ID GPL4133
Series (2)
GSE18438 Over-expression of hsa-miR-221 in JSC1 cells and identification of miRNA targets expressed in a panel of B-cell lines
GSE18439 Transcription repression of miR-221 leads to p27 expression in PEL

Data table header descriptions
ID_REF
VALUE Scale normalised log2 ratio (sample/reference)

Data table
ID_REF VALUE
1 -0.147856489915322
2 -0.0666250795011545
3 -0.0713804789600905
4 -0.075736919677117
5 -0.0797227598518824
6 -0.0832994863159713
7 -0.0865477054203663
8 -0.0894468824505983
9 -0.0920300857492899
10 -0.0943177236103546
11 -0.0962864735765954
12 1.50063900764548
13 2.60887738647124
14 1.04172981504222
15 -1.88071555804267
16 1.2912881699831
17 0.420861056597708
18 -1.03276213893647
19 1.22073814236539
20 2.61506036761276

Total number of rows: 45015

Table truncated, full table size 1047 Kbytes.




Supplementary file Size Download File type/resource
GSM459476_US45102835_251485046356_S01_GE2-v5_95_Feb07_1_3.txt.gz 14.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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