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Sample GSM459486 Query DataSets for GSM459486
Status Public on Dec 05, 2015
Title BCP1_Bcell_panel_rep2
Sample type RNA
 
Channel 1
Source name BCP1_Bcell_panel
Organism Homo sapiens
Characteristics cell line: BCP1
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy5
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
Channel 2
Source name Stratagene reference RNA
Organism Homo sapiens
Characteristics sample: reference RNA
hairpin: none
Extracted molecule total RNA
Extraction protocol Trizol purification of total RNA
Label Cy3
Label protocol Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
 
 
Hybridization protocol Hybridization was performed for 17 hrs at 65°C
Scan protocol Arrays were scanned on an Agilent DNA microarray scanner. Images were analyzed and data were extracted using the standard procedures of Agilent Feature Extraction software (v9.5.3.1).
Description Sample used in panel of B-cell lines
Data processing Scale normalisation across arrays (R/BioConductor), with samples 1-6 and 7-20 being normalised separately.
 
Submission date Oct 06, 2009
Last update date Dec 05, 2015
Contact name Dan Frampton
Organization name UCL
Department Infection
Lab Kellam
Street address Gower Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
 
Platform ID GPL4133
Series (2)
GSE18438 Over-expression of hsa-miR-221 in JSC1 cells and identification of miRNA targets expressed in a panel of B-cell lines
GSE18439 Transcription repression of miR-221 leads to p27 expression in PEL

Data table header descriptions
ID_REF
VALUE Scale normalised log2 ratio (sample/reference)

Data table
ID_REF VALUE
1 0.856524684056228
2 -0.0939099912316214
3 0.140469787855838
4 2.73957594076313
5 -0.0999252667949502
6 -0.101514040167130
7 -0.102867098358490
8 -0.103936762381392
9 -0.104835089386932
10 -0.105493428422981
11 -1.50963158856115
12 -2.03447287560586
13 2.04364772457229
14 0.773752639678299
15 -1.25429050913097
16 -1.51052778104841
17 -2.35408106710277
18 0.232192970601985
19 0.172759042632682
20 1.61601681806185

Total number of rows: 45015

Table truncated, full table size 1051 Kbytes.




Supplementary file Size Download File type/resource
GSM459486_BCP1b.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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