GEO DataSets

Analysis of estrogen receptor (ER) alpha positive MCF-7 breast cancer cells overexpressing constitutively active Raf-1, constitutively active MEK, constitutively active c-erbB-2, or ligand-activatable EGFR. Results indicate that increased MAPK activation results in loss of ER-alpha expression.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 6 cell line sets

Platform:

GPL96

Series:

GSE3542

18 Samples

Download data: CEL

(Submitter supplied) Profiling of MCF-7 cell lines stably overexpressing constitutively active Raf-1, constitutively active MEK, constitutively active c-erbB-2, or ligand-activatable EGFR as models of overexpressed growth factor signaling, as well as control vector transfected cells (coMCF-7) and control vector transfected cells long-term adapted for estrogen-independent growth (coMCF-7/lt-E2). Keywords: Cell Line Comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1925

Platform:

GPL96

18 Samples

Download data: CEL

(Submitter supplied) Estrogen receptor-{alpha} (ER{alpha}) and its ligand estradiol play critical roles in breast cancer growth and are important therapeutic targets for this disease. Using chromatin immunoprecipitation (ChIP)-on-chip, ligand-bound ER{alpha} was recently found to function as a master transcriptional regulator via binding to many cis-acting sites genome-wide. Here, we used an alternative technology (ChIP cloning) and identified 94 ER{alpha} target loci in breast cancer cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3283

Platform:

GPL570

9 Samples

Download data: CEL

Analysis of MCF-7 breast cancer cells treated with estradiol for 3 or 6 hours. Results combined with ChIP experiments to identify estrogen receptor alpha binding sites and estradiol target genes in breast cancer cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 time sets

Platform:

GPL570

Series:

GSE11506

9 Samples

Download data: CEL

(Submitter supplied) Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0·01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1326

Platform:

GPL96

12 Samples

Download data

Analysis of the response of estrogen receptor (ER) negative MDA-MB-231 breast cancer cells infected with full-length ER alpha adenoviral constructs to treatment with 17beta-estradiol (E2). Results provide insight into the anti-proliferative effect of E2 on breast cancer cells reexpressing ER.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 protocol sets

Platform:

GPL96

Series:

GSE2251

12 Samples

Download data

(Submitter supplied) Clinical heterogeneity of esrtrogen receptor-negative, progesterone receptor-negative [ER(-)/PR(-)] breast cancer (BC) suggests biological heterogeneity. We performed gene expression analysis of primary BCs and BC cell lines to identify the underlying biology of ER(-)/PR(-) disease, define subsets, and identify potential therapeutic targets.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

105 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL10558 GPL571

22 Samples

Download data: CEL

(Submitter supplied) Anlaysis of the differential gene expression between T47D cells expressing wild type (WT) progesterone receptor isoform B (PR) or SUMOylation-deficient PR molecules.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: TXT

(Submitter supplied) Anlaysis of the differential gene expression between T47D cells expressing wild type (WT) progesterone receptor isoform B (PR) or SUMOylation-deficient PR molecules.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

10 Samples

Download data: CEL

(Submitter supplied) The nuclear hormone receptor, estrogen receptor-alpha (ERα), and MAP kinases both play key roles in hormone-dependent cancers, yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERα activates and interacts with ERK2, a downstream effector in the MAPK pathway, resulting in ERK2 and ERα colocalization at chromatin binding sites across the genome of breast cancer cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4052

Platform:

GPL571

30 Samples

Download data: CEL

Analysis of MCF7 cells depleted of ERK1 or ERK2 and treated with estradiol for up to 24hrs. ERK1 and ERK2 are downstream effectors in the MAP kinase pathway; estrogen receptor α (ERα) and MAPKs play key roles in hormone-dependent cancers. Results provide insight into ERα and MAPK interactions.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 genotype/variation, 2 time sets

Platform:

GPL571

Series:

GSE24592

30 Samples

Download data: CEL

(Submitter supplied) We generated DNA microarray based gene expression profiles from three estrogen receptor a (ERa) positive breast cancer cell lines stimulated by 17ß-estradiol (E2) in vitro over a time course, as well as from MCF-7 cells grown as xenografts in ovariectomized athymic nude mice with E2 supplementation and after its withdrawal. Keywords: Cell line and xenograft comparisons

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL96

47 Samples

Download data

(Submitter supplied) Cancer cell motility and invasiveness are fundamental characteristics of the malignant phenotype and are regulated through diverse signaling networks involving kinases and transcription factors. In this study, we identify a nuclear hormone receptor (ERα)-protein kinase (ERK5)-cofilin (CFL1) network that specifies the degree of breast cancer cell aggressiveness through coupling of actin reorganization and hormone receptor-mediated transcription. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5026

Platform:

GPL96

8 Samples

Download data: CEL

Analysis of MCF-7 breast cancer (BC) cells depleted for ERK5 then treated with 17β-estradiol. Increased kinase activity is associated with a poorer outcome for BC patients. Results provide insight into the interplay between estrogen receptor and protein kinase pathways to regulate BC aggressiveness

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL96

Series:

GSE53394

8 Samples

Download data: CEL

(Submitter supplied) Estrogen receptor alpha (ERα) expression in breast cancer is predictive of response to endocrine therapy, however resistance is common in ERα-positive tumors that over-express the growth factor receptor ERBB2. Even in the absence of estrogen, ERα can be activated by growth factors including the epidermal growth factor (EGF). EGF induces a transcriptional program distinct from estrogen, however the mechanism of the stimulus-specific response is unknown. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by genome tiling array; Expression profiling by array

8 related Platforms

47 Samples

Download data: BAR, CEL

(Submitter supplied) Estrogen receptor alpha (ERα)-positive breast cancers, while initially being responsive, eventually develop resistance to ERα targeted therapies through ERα-dependent and ERα-independent mechanisms. Through functional genomic studies we report heretofore unrecognized role for the axon guidance dependence receptor UNC5A in fine-tuning estradiol (E2) and anti-estrogen response. Knockdown of the estradiol-inducible UNC5A caused unrestricted ERα signaling as evident from deregulated E2-regulated gene expression and E2-independent tumorigenesis accompanied with multi-organ metastatic spread in xenograft models. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16288

16 Samples

Download data: TXT, XLS

(Submitter supplied) Our research data suggest that almost all basal-like tumors have an EGFR-activation profile, however, few may respond to the direct inhibition of EGFR due to ligand independent activation of the EGFR-RAS-MEK pathway via CRYAB or KRAS function. Thus, for those tumors that show a ligand independent EGFR-activation profile, alternative strategies that target downstream components like MEK may prove to be viable alternatives. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL1390 GPL887

191 Samples

Download data

(Submitter supplied) Orphan nuclear receptor estrogen-related receptor alpha (ERRα) has recently been shown to carry negative prognostic significance in breast and ovarian cancers. The specific role of ERRα in tumor growth and progression, however, is yet to be fully understood. The significant homology between estrogen receptor alpha (ERα) and ERRα initially suggested that these receptors may share similar transcriptional targets. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

23 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9052 GPL16791

26 Samples

Download data: WIG