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Series GSE24592 Query DataSets for GSE24592
Status Public on Jan 10, 2011
Title Genomic Collaboration of Estrogen Receptor-α and ERK2 in Regulating Gene and Proliferation Programs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The nuclear hormone receptor, estrogen receptor-alpha (ERα), and MAP kinases both play key roles in hormone-dependent cancers, yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERα activates and interacts with ERK2, a downstream effector in the MAPK pathway, resulting in ERK2 and ERα colocalization at chromatin binding sites across the genome of breast cancer cells.
KEYWORDS: siRNA knock-down, ligand treatment
Overall design MCF-7 human breast adenocarcinoma cells were tranfected with control, ERK1 and ERK2 siRNA for 60 hours and treated with 0.1% EtOH (Vehicle) or 10 nM E2 for 4 hours or 24 hours, and cDNA microarray analyses were carried out using Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array.
Contributor(s) Erdogan ZM, Katzenellenbogen B
Citation(s) 20956553
Submission date Oct 08, 2010
Last update date Dec 06, 2018
Contact name Zeynep Madak Erdogan
Organization name UIUC
Department FSHN
Lab Zeynep Madak Erdogan
Street address 1201 S Goodwin Ave
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (30)
GSM606107 siCtrlVeh4h_2649
GSM606108 siCtrlVeh4h_2663
GSM606109 siCtrlVeh4h_2657
BioProject PRJNA132379

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Supplementary file Size Download File type/resource
GSE24592_RAW.tar 57.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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