 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 08, 2006 |
| Title |
Cross-hybridization comparison between cRNA target and cDNA target |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We used two RNA samples that differed only by a set of three hemoglobin transcripts to compare microarray cross-hybridization between two target preparation protocols. We found widespread cross-hybridization using standard cRNA target but substantially less cross-hybridization using cDNA target.
Keywords: protocol comparison
|
| |
|
| Overall design |
2 protocols x 2 RNA samples x 3 replicates = 12 arrays.
|
| |
|
| Contributor(s) |
Eklund AC, Turner LR, Chen P, Jensen RV, deFeo G, Kopf-Sill AR, Szallasi Z |
| Citation(s) |
16964210 |
| |
| Submission date |
Mar 24, 2006 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Aron C Eklund |
| Organization name |
Technical University of Denmark
|
| Department |
Center for Biological Sequence Analysis
|
| Street address |
Kemitorvet, Building 208
|
| City |
Lyngby |
| ZIP/Postal code |
2800 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (12)
|
| GSM101572 |
Jurkat+Globin, cRNA target, replicate 1 |
| GSM101573 |
Jurkat+Globin, cRNA target, replicate 2 |
| GSM101574 |
Jurkat+Globin, cRNA target, replicate 3 |
| GSM101575 |
Jurkat, cDNA target, replicate 1 |
| GSM101576 |
Jurkat, cDNA target, replicate 2 |
| GSM101577 |
Jurkat, cDNA target, replicate 3 |
| GSM101578 |
Jurkat+Globin, cDNA target, replicate 1 |
| GSM101579 |
Jurkat+Globin, cDNA target, replicate 2 |
| GSM101580 |
Jurkat+Globin, cDNA target, replicate 3 |
|
| Relations |
| BioProject |
PRJNA94457 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4532_RAW.tar |
38.9 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
|
|
|
|
 |
Less...
More...