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Sample GSM101570 Query DataSets for GSM101570
Status Public on Sep 08, 2006
Title Jurkat, cRNA target, replicate 2
Sample type RNA
Source name Jurkat cells
Organism Homo sapiens
Characteristics Tissue: T-lymphocyte leukemia cell line
Biomaterial provider Affymetrix
Extracted molecule total RNA
Label biotin
Label protocol Biotinylated cRNA was prepared using the MessageAmp II kit (Ambion).
Hybridization protocol cRNA was hybridized according to the GeneChip Expression Analysis Technical Manual (rev. 4, Affymetrix). Briefly, cRNA was hybridized at 50 ng/ul for 16 hours at 45 C.
Scan protocol Arrays were scanned using a GeneChip Scanner 3000.
Description Jurkat RNA, from GeneChip Globin-Reduction RNA Controls (Affymetrix P/N 900586)
Data processing The data were analyzed with the MAS 5 algorithm in the R statistical environment using default settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Mar 24, 2006
Last update date Nov 04, 2009
Contact name Aron C Eklund
Organization name Technical University of Denmark
Department Center for Biological Sequence Analysis
Street address Kemitorvet, Building 208
City Lyngby
ZIP/Postal code 2800
Country Denmark
Platform ID GPL571
Series (1)
GSE4532 Cross-hybridization comparison between cRNA target and cDNA target

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)

Data table
1007_s_at 59.05 P
1053_at 113.49 P
117_at 37.55 P
121_at 42.5 P
1255_g_at 0.57 A
1294_at 9.53 P
1316_at 4.76 A
1320_at 0.6 A
1405_i_at 0.12 A
1431_at 36.32 P
1438_at 0.64 A
1487_at 50.07 P
1494_f_at 6.94 P
1598_g_at 17.48 A
160020_at 5.08 A
1729_at 40.82 P
1773_at 17.17 A
177_at 5.7 P
179_at 26.55 P
1861_at 45.27 P

Total number of rows: 22277

Table truncated, full table size 407 Kbytes.

Supplementary file Size Download File type/resource
GSM101570.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM101570.EXP.gz 490 b (ftp)(http) EXP

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