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Sample GSM101572 Query DataSets for GSM101572
Status Public on Sep 08, 2006
Title Jurkat+Globin, cRNA target, replicate 1
Sample type RNA
Source name Jurkat cells, hemoglobin spikes added
Organism Homo sapiens
Characteristics Tissue: T-lymphocyte leukemia cell line
Biomaterial provider Affymetrix
Extracted molecule total RNA
Label biotin
Label protocol Biotinylated cRNA was prepared using the MessageAmp II kit (Ambion).
Hybridization protocol cRNA was hybridized according to the GeneChip Expression Analysis Technical Manual (rev. 4, Affymetrix). Briefly, cRNA was hybridized at 50 ng/ul for 16 hours at 45 C.
Scan protocol Arrays were scanned using a GeneChip Scanner 3000.
Description Jurkat + Globin RNA, from GeneChip Globin-Reduction RNA Controls (Affymetrix P/N 900586)
Data processing The data were analyzed with the MAS 5 algorithm in the R statistical environment using default settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Mar 24, 2006
Last update date Nov 04, 2009
Contact name Aron C Eklund
Organization name Technical University of Denmark
Department Center for Biological Sequence Analysis
Street address Kemitorvet, Building 208
City Lyngby
ZIP/Postal code 2800
Country Denmark
Platform ID GPL571
Series (1)
GSE4532 Cross-hybridization comparison between cRNA target and cDNA target

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)

Data table
1007_s_at 104.96 P
1053_at 82.14 P
117_at 39.73 P
121_at 120.42 P
1255_g_at 0.46 A
1294_at 7.62 A
1316_at 11.71 P
1320_at 1.25 A
1405_i_at 0.17 A
1431_at 46.62 P
1438_at 1.06 A
1487_at 46.75 M
1494_f_at 10.68 A
1598_g_at 41.5 A
160020_at 31.66 A
1729_at 7.96 A
1773_at 22.11 A
177_at 10.78 P
179_at 80.14 A
1861_at 38.88 P

Total number of rows: 22277

Table truncated, full table size 407 Kbytes.

Supplementary file Size Download File type/resource
GSM101572.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM101572.EXP.gz 342 b (ftp)(http) EXP

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