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Status |
Public on Sep 08, 2006 |
Title |
Jurkat+Globin, cDNA target, replicate 3 |
Sample type |
RNA |
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|
Source name |
Jurkat cells, hemoglobin spikes added
|
Organism |
Homo sapiens |
Characteristics |
Tissue: T-lymphocyte leukemia cell line
|
Biomaterial provider |
Affymetrix
|
Extracted molecule |
total RNA |
Label |
biotin
|
Label protocol |
Biotinylated cDNA was prepared using the Ovation Biotin System (NuGEN Technologies, Inc.) from 50 ng starting RNA.
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Hybridization protocol |
cDNA was hybridized according to the Ovation Biotin RNA Amplification and Labeling System User Guide (rev. 1, NuGEN Technologies). Briefly, cDNA was hybridized at 10 ng/ul for 18 hours at 45 C.
|
Scan protocol |
Arrays were scanned using a GeneChip Scanner 3000.
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Description |
Jurkat + Globin RNA, from GeneChip Globin-Reduction RNA Controls (Affymetrix P/N 900586)
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Data processing |
The data were analyzed with the MAS 5 algorithm in the R statistical environment using default settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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Submission date |
Mar 24, 2006 |
Last update date |
Nov 04, 2009 |
Contact name |
Aron C Eklund |
Organization name |
Technical University of Denmark
|
Department |
Center for Biological Sequence Analysis
|
Street address |
Kemitorvet, Building 208
|
City |
Lyngby |
ZIP/Postal code |
2800 |
Country |
Denmark |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE4532 |
Cross-hybridization comparison between cRNA target and cDNA target |
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