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Sample GSM101576 Query DataSets for GSM101576
Status Public on Sep 08, 2006
Title Jurkat, cDNA target, replicate 2
Sample type RNA
Source name Jurkat cells
Organism Homo sapiens
Characteristics Tissue: T-lymphocyte leukemia cell line
Biomaterial provider Affymetrix
Extracted molecule total RNA
Label biotin
Label protocol Biotinylated cDNA was prepared using the Ovation Biotin System (NuGEN Technologies, Inc.) from 50 ng starting RNA.
Hybridization protocol cDNA was hybridized according to the Ovation Biotin RNA Amplification and Labeling System User Guide (rev. 1, NuGEN Technologies). Briefly, cDNA was hybridized at 10 ng/ul for 18 hours at 45 C.
Scan protocol Arrays were scanned using a GeneChip Scanner 3000.
Description Jurkat RNA, from GeneChip Globin-Reduction RNA Controls (Affymetrix P/N 900586)
Data processing The data were analyzed with the MAS 5 algorithm in the R statistical environment using default settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Mar 24, 2006
Last update date Nov 04, 2009
Contact name Aron C Eklund
Organization name Technical University of Denmark
Department Center for Biological Sequence Analysis
Street address Kemitorvet, Building 208
City Lyngby
ZIP/Postal code 2800
Country Denmark
Platform ID GPL571
Series (1)
GSE4532 Cross-hybridization comparison between cRNA target and cDNA target

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)

Data table
1007_s_at 13 P
1053_at 77.14 P
117_at 16.08 P
121_at 0.46 A
1255_g_at 0.14 A
1294_at 3.38 A
1316_at 5.87 P
1320_at 3.32 A
1405_i_at 0.13 A
1431_at 15.36 P
1438_at 0.55 A
1487_at 41.02 P
1494_f_at 0.54 A
1598_g_at 2.35 A
160020_at 3.62 A
1729_at 7.29 M
1773_at 4.12 A
177_at 6.4 P
179_at 0.79 A
1861_at 10.08 P

Total number of rows: 22277

Table truncated, full table size 405 Kbytes.

Supplementary file Size Download File type/resource
GSM101576.CEL.gz 3.1 Mb (ftp)(http) CEL
GSM101576.EXP.gz 502 b (ftp)(http) EXP

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