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Series GSE37040 Query DataSets for GSE37040
Status Public on Jan 22, 2013
Title Expression data from human pluripotent stem cells and differentiated cells treated with pluripotent-specific inhibitors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The use of human pluripotent stem cells (hPSCs) in cell therapy is hindered by the tumorigenic risk from residual undifferentiated cells. Here we performed a high-throughput screen of over 52,000 small molecules, and identified 15 highly selective cytotoxic inhibitors of hPSCs (PluriSIns). Cellular and molecular analyses revealed that the most selective compound, PluriSIn #1, is a pluripotent-specific inhibitor of stearoyl-coA desaturase (SCD1), the key enzyme in the biosynthesis of monounsaturated fatty acids (MUFA). SCD1 inhibition in hPSCs induced ER stress, protein synthesis attenuation, and apoptosis of these cells, revealing that MUFA biosynthesis is crucial for their survival. PluriSIn #1 was also cytotoxic toward the ICM cells of mouse embryos, indicating that the dependence on SCD1 is inherent to the pluripotent state. Finally, application of PluriSIn #1 prevented teratoma formation from tumorigenic undifferentiated cells. Our novel method to eliminate undifferentiated cells from culture should thus increase the safety of hPSC-based treatments.
Overall design Expression data from undifferentiated and differentiated human embryonic stem cells. Total RNA was isolated from undifferentiated human pluripotent stem cells grown on matrigel with mTeSR1 medium, or from early endodermal progenitor cells differentiated from human embryonic stem cells.
Contributor(s) Ben-David U, Benvenisty N
Citation(s) 23318055
Submission date Apr 04, 2012
Last update date Dec 06, 2018
Contact name Uri Ben-David
Phone 97226585185
Organization name Hebrew University
Department Genetics
Lab Nissim Benvenisty
Street address Givat-Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (7)
GSM909270 H9 control (1)
GSM909271 H9 control (2)
GSM909272 H9 PluriSIn #1 (1)
BioProject PRJNA157975

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37040_RAW.tar 13.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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