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Sample GSM909272 Query DataSets for GSM909272
Status Public on Jan 22, 2013
Title H9 PluriSIn #1 (1)
Sample type RNA
 
Source name H9, PluriSIn #1
Organism Homo sapiens
Characteristics cell type: undifferentiated human embryonic stem cells
genotype/variation: 46(X,X)
age: passage 52
cell line: H9
treatment: PluriSIn #1
Treatment protocol Undifferentiated pluripotent stem cells were grown on matrigel with mTeSR1 and exposed to PluriSIn #1 at 20uM with 0.02% DMSO for 12 h.
Growth protocol Cells were grown on matrigel-coated plates using mTeSR1 defined medium.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using 5 Prime RNA isolation kit.
Label biotin
Label protocol 200ng total RNA, Affymetrix protocol.
 
Hybridization protocol Affymetrix protocol, 11ug cRNA.
Scan protocol GeneChip scanner 3000 7G, GeneChip Operating Software v1.4.
Description Gene expression data from undifferentiated pluripotent stem cells grown on matrigel with mTeSR1 and exposed to PluriSIn #1 at 20uM with 0.02% DMSO for 12 h.
Data processing Affymetrix Expression Console MAS5.
 
Submission date Apr 04, 2012
Last update date May 22, 2014
Contact name Uri Ben-David
E-mail(s) uri.bendavid@gmail.com
Phone 97226585185
Organization name Hebrew University
Department Genetics
Lab Nissim Benvenisty
Street address Givat-Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
 
Platform ID GPL571
Series (1)
GSE37040 Expression data from human pluripotent stem cells and differentiated cells treated with pluripotent-specific inhibitors

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 934.1443
1053_at 888.6741
117_at 20.34453
121_at 76.39984
1255_g_at 247.1676
1294_at 21.36477
1316_at 60.52642
1320_at 45.81792
1405_i_at 0.8497469
1431_at 10.98386
1438_at 4.491335
1487_at 237.7963
1494_f_at 31.94054
1598_g_at 180.8289
160020_at 122.7407
1729_at 21.42532
177_at 23.34814
1773_at 33.09404
179_at 61.25303
1861_at 94.83868

Total number of rows: 22215

Table truncated, full table size 429 Kbytes.




Supplementary file Size Download File type/resource
GSM909272_H9_PluriSIn__1_1_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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