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Sample GSM909271 Query DataSets for GSM909271
Status Public on Jan 22, 2013
Title H9 control (2)
Sample type RNA
 
Source name H9, control
Organism Homo sapiens
Characteristics cell type: undifferentiated human embryonic stem cells
genotype/variation: 46(X,X)
age: passage 52
cell line: H9
treatment: DMSO vehicle
Treatment protocol Undifferentiated pluripotent stem cells were grown on matrigel with mTeSR1 and exposed to 0.02% DMSO for 12 h.
Growth protocol Cells were grown on matrigel-coated plates using mTeSR1 defined medium.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using 5 Prime RNA isolation kit.
Label biotin
Label protocol 200ng total RNA, Affymetrix protocol.
 
Hybridization protocol Affymetrix protocol, 11ug cRNA.
Scan protocol GeneChip scanner 3000 7G, GeneChip Operating Software v1.4.
Description Gene expression data from undifferentiated pluripotent stem cells grown on matrigel with mTeSR1 and exposed to 0.02% DMSO for 12 h.
Data processing Affymetrix Expression Console MAS5.
 
Submission date Apr 04, 2012
Last update date May 22, 2014
Contact name Uri Ben-David
E-mail(s) uri.bendavid@gmail.com
Phone 97226585185
Organization name Hebrew University
Department Genetics
Lab Nissim Benvenisty
Street address Givat-Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
 
Platform ID GPL571
Series (2)
GSE37040 Expression data from human pluripotent stem cells and differentiated cells treated with pluripotent-specific inhibitors
GSE57909 Expression data from human pluripotent stem cells treated with PluriSIn#2

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1023.974
1053_at 1035.981
117_at 24.72454
121_at 94.02959
1255_g_at 206.4856
1294_at 11.4283
1316_at 27.13181
1320_at 25.72162
1405_i_at 1.498916
1431_at 10.43848
1438_at 3.634173
1487_at 245.8593
1494_f_at 33.04127
1598_g_at 182.2412
160020_at 113.7621
1729_at 37.95407
177_at 27.54143
1773_at 30.1053
179_at 88.79823
1861_at 113.6772

Total number of rows: 22215

Table truncated, full table size 430 Kbytes.




Supplementary file Size Download File type/resource
GSM909271_H9_control_2_.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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