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Sample GSM909270 Query DataSets for GSM909270
Status Public on Jan 22, 2013
Title H9 control (1)
Sample type RNA
 
Source name H9, control
Organism Homo sapiens
Characteristics cell type: undifferentiated human embryonic stem cells
genotype/variation: 46(X,X)
age: passage 52
cell line: H9
treatment: DMSO vehicle
Treatment protocol Undifferentiated pluripotent stem cells were grown on matrigel with mTeSR1 and exposed to 0.02% DMSO for 12 h.
Growth protocol Cells were grown on matrigel-coated plates using mTeSR1 defined medium.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using 5 Prime RNA isolation kit.
Label biotin
Label protocol 200ng total RNA, Affymetrix protocol.
 
Hybridization protocol Affymetrix protocol, 11ug cRNA.
Scan protocol GeneChip scanner 3000 7G, GeneChip Operating Software v1.4.
Description Gene expression data from undifferentiated pluripotent stem cells grown on matrigel with mTeSR1 and exposed to 0.02% DMSO for 12 h.
Data processing Affymetrix Expression Console MAS5.
 
Submission date Apr 04, 2012
Last update date May 22, 2014
Contact name Uri Ben-David
E-mail(s) uri.bendavid@gmail.com
Phone 97226585185
Organization name Hebrew University
Department Genetics
Lab Nissim Benvenisty
Street address Givat-Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
 
Platform ID GPL571
Series (2)
GSE37040 Expression data from human pluripotent stem cells and differentiated cells treated with pluripotent-specific inhibitors
GSE57909 Expression data from human pluripotent stem cells treated with PluriSIn#2

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 884.2045
1053_at 952.2374
117_at 14.77808
121_at 84.86877
1255_g_at 312.0287
1294_at 26.46573
1316_at 63.37922
1320_at 31.07742
1405_i_at 1.582665
1431_at 11.0954
1438_at 18.80867
1487_at 244.113
1494_f_at 21.99428
1598_g_at 196.7211
160020_at 116.8702
1729_at 41.27198
177_at 28.51656
1773_at 24.11048
179_at 60.40866
1861_at 71.62231

Total number of rows: 22215

Table truncated, full table size 429 Kbytes.




Supplementary file Size Download File type/resource
GSM909270_H9_control_1_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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