|Public on Sep 02, 2013
|Role of Per2 in the control of liver glycogen metabolism
|Expression profiling by array
|To investigate the role of Per2 in glucose metabolism in-vivo we used mice bearing a targeted gene mutation in the Per2 gene (Per2brdm) and thus unable to express a functional Per2 protein. Mice were hosted in our standard mouse facility in a 12-hour light and 12-hour dark cycle. Wt and Per2brdm mice were fed with either standard chow diet or high-fat diet for 24 weeks and analyzed for glucose homeostasis. Glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed on mice food deprived for 7 hours. For pyruvate tolerance test (PTT) mice were fasted for 14 hours. Glucose levels were measured over 12 hours starvation time-courses during the light and the dark phase. Fed and fasting blood glucose and hepatic glycogen content were measured over different circadian time-points. To investigate the role of Per2 in the control of liver gene expression we performed DNA-microarray analysis of RNA preparations from liver of Per2brdm and WT mice.
|We extracted total RNA from liver of four different Per2brdm and WT mice. Mice from the same cohort were sacrificed after 8 hours of starvation. After normalization we performed a fold change comparison selecting probes with a p value < 0.05.
|Zani F, Becattini B, Provenzani A, Stauffer A, Montani J, Albrecht U, Solinas G
|Jun 22, 2011
|Last update date
|Jan 12, 2017
|University of Trento
|Laboratory of Genomic Screening
|via delle regole 101
|Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)