GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM745914 Query DataSets for GSM745914
Status Public on Sep 02, 2013
Title Liver_mPER2_KO_rep1
Sample type RNA
Source name Liver
Organism Mus musculus
Characteristics strain: mixed background 129sv and C57Bl/6
tissue: liver
genotype/variation: mper2brdm1 mutant (PMID: 10408444)
Extracted molecule total RNA
Extraction protocol Standard single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Label Cyanine 3-CTP
Label protocol Standard Agilent protocol that uses T7 RNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3-labeled CTP (Manual:One-Color Microarray-Based Gene Expression Analysis)
Hybridization protocol Standard Agilent protocol using suggested blocking agent, fragmentation buffer and hybridization buffer. Hybridization run for 17 hours at 65°C. (Manual:One-Color Microarray-Based Gene Expression Analysis)
Scan protocol Scanning was performed using Agilnet scanner at 5 uM resolution, green dye channel
Data processing Signal was extracted using feature expression and data were nornalized to the 75th percentile of of all of non-control probes on the microarray using genespring software
Submission date Jun 21, 2011
Last update date Sep 02, 2013
Contact name Alessandro Provenzani
Phone +390461283094
Organization name University of Trento
Department CIBIO
Lab Laboratory of Genomic Screening
Street address via delle regole 101
City Trento
ZIP/Postal code 38100
Country Italy
Platform ID GPL7202
Series (1)
GSE30139 Role of Per2 in the control of liver glycogen metabolism

Data table header descriptions
RAW Raw data
VALUE Normalized data
PMA Present/Marginal/Absent

Data table
GE_BrightCorner 78361.34 1.0311732 P
DarkCorner 8.217814 0.3214426 A
A_52_P616356 15.60879 0.89690113 A
A_52_P580582 42.975307 0.33170366 P
A_52_P403405 33.08074 0.9651656 P
A_52_P819156 31.294357 0.9958458 A
A_51_P331831 146.70361 0.11653805 P
A_51_P430630 15.256601 0.80287504 A
A_52_P502357 10.352599 0.63147306 A
A_52_P299964 24.20653 1.3258314 A
A_51_P356389 14.86693 0.44015884 A
A_52_P684402 403.96426 -0.07214975 P
A_51_P414208 35.78295 -0.6476908 P
A_51_P280918 631.16284 -0.24077749 P
A_52_P613688 29.376717 0.17023373 A
A_52_P258194 46.20603 1.2724285 P
A_52_P229271 49.48463 -0.79308295 P
A_52_P214630 188.44337 0.8146391 P
A_52_P579519 183.74371 -0.4567294 P
A_52_P979997 14.177831 0.7404146 A

Total number of rows: 41252

Table truncated, full table size 1437 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap