NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM745916 Query DataSets for GSM745916
Status Public on Sep 02, 2013
Title Liver_mPER2_KO_rep3
Sample type RNA
 
Source name Liver
Organism Mus musculus
Characteristics strain: mixed background 129sv and C57Bl/6
tissue: liver
genotype/variation: mper2brdm1 mutant (PMID: 10408444)
Extracted molecule total RNA
Extraction protocol Standard single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Label Cyanine 3-CTP
Label protocol Standard Agilent protocol that uses T7 RNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3-labeled CTP (Manual:One-Color Microarray-Based Gene Expression Analysis)
 
Hybridization protocol Standard Agilent protocol using suggested blocking agent, fragmentation buffer and hybridization buffer. Hybridization run for 17 hours at 65°C. (Manual:One-Color Microarray-Based Gene Expression Analysis)
Scan protocol Scanning was performed using Agilnet scanner at 5 uM resolution, green dye channel
Data processing Signal was extracted using feature expression and data were nornalized to the 75th percentile of of all of non-control probes on the microarray using genespring software
 
Submission date Jun 21, 2011
Last update date Sep 02, 2013
Contact name Alessandro Provenzani
E-mail(s) alessandro.provenzani@unitn.it
Phone +390461283094
Organization name University of Trento
Department CIBIO
Lab Laboratory of Genomic Screening
Street address via delle regole 101
City Trento
ZIP/Postal code 38100
Country Italy
 
Platform ID GPL7202
Series (1)
GSE30139 Role of Per2 in the control of liver glycogen metabolism

Data table header descriptions
ID_REF
RAW Raw data
VALUE Normalized data
PMA Present/Marginal/Absent

Data table
ID_REF RAW VALUE PMA
GE_BrightCorner 45069.137 -0.14948416 P
DarkCorner 11.323508 0.40128136 A
A_52_P616356 20.15582 0.8830838 A
A_52_P580582 49.42897 0.1508975 P
A_52_P403405 28.144873 0.34939098 A
A_52_P819156 38.641365 0.9174342 A
A_51_P331831 162.47751 -0.11878157 P
A_51_P430630 23.187662 1.0241437 A
A_52_P502357 16.132238 0.88877153 A
A_52_P299964 30.00897 1.2531738 A
A_51_P356389 22.570307 0.65982294 A
A_52_P684402 563.94995 0.026534557 P
A_51_P414208 28.667114 -1.3502216 A
A_51_P280918 814.9682 -0.25470114 P
A_52_P613688 48.91563 0.523201 P
A_52_P258194 43.395504 0.7992377 A
A_52_P229271 118.10501 0.07927966 P
A_52_P214630 214.71153 0.6202526 P
A_52_P579519 260.0173 -0.3384719 P
A_52_P979997 9.429692 -0.23059511 A

Total number of rows: 41252

Table truncated, full table size 1437 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap