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Sample GSM745920 Query DataSets for GSM745920
Status Public on Sep 02, 2013
Title Liver_WT_rep3
Sample type RNA
 
Source name Liver
Organism Mus musculus
Characteristics strain: mixed background 129sv and C57Bl/6
tissue: liver
genotype/variation: WT
Extracted molecule total RNA
Extraction protocol Standard single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Label Cyanine 3-CTP
Label protocol Standard Agilent protocol that uses T7 RNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3-labeled CTP (Manual:One-Color Microarray-Based Gene Expression Analysis)
 
Hybridization protocol Standard Agilent protocol using suggested blocking agent, fragmentation buffer and hybridization buffer. Hybridization run for 17 hours at 65°C. (Manual:One-Color Microarray-Based Gene Expression Analysis)
Scan protocol Scanning was performed using Agilnet scanner at 5 uM resolution, green dye channel
Data processing Signal was extracted using feature expression and data were nornalized to the 75th percentile of of all of non-control probes on the microarray using genespring software
 
Submission date Jun 21, 2011
Last update date Sep 02, 2013
Contact name Alessandro Provenzani
E-mail(s) alessandro.provenzani@unitn.it
Phone +390461283094
Organization name University of Trento
Department CIBIO
Lab Laboratory of Genomic Screening
Street address via delle regole 101
City Trento
ZIP/Postal code 38100
Country Italy
 
Platform ID GPL7202
Series (1)
GSE30139 Role of Per2 in the control of liver glycogen metabolism

Data table header descriptions
ID_REF
RAW Raw data
VALUE Normalized data
PMA Present/Marginal/Absent

Data table
ID_REF RAW VALUE PMA
GE_BrightCorner 57728.367 0.23583746 P
DarkCorner 4.9891376 -0.75299597 A
A_52_P616356 8.147618 -0.3954811 A
A_52_P580582 51.4125 0.2358408 P
A_52_P403405 8.321497 -1.3803854 A
A_52_P819156 10.834222 -0.8889351 A
A_51_P331831 170.6726 -0.019608974 P
A_51_P430630 4.5733986 -1.2896938 A
A_52_P502357 4.5744553 -0.90132236 A
A_52_P299964 8.097592 -0.6084738 A
A_51_P356389 11.89552 -0.2360034 A
A_52_P684402 604.1236 0.15399218 P
A_51_P414208 333.42642 2.2178593 P
A_51_P280918 998.7731 0.0668931 P
A_52_P613688 21.464663 -0.63695 A
A_52_P258194 15.0815115 -0.69734573 A
A_52_P229271 103.76571 -0.07927966 P
A_52_P214630 88.34228 -0.6327901 P
A_52_P579519 356.63486 0.1455493 P
A_52_P979997 6.038269 -0.845489 A

Total number of rows: 41252

Table truncated, full table size 1446 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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