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Sample GSM745921 Query DataSets for GSM745921
Status Public on Sep 02, 2013
Title Liver_WT_rep4
Sample type RNA
 
Source name Liver
Organism Mus musculus
Characteristics strain: mixed background 129sv and C57Bl/6
tissue: liver
genotype/variation: WT
Extracted molecule total RNA
Extraction protocol Standard single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Label Cyanine 3-CTP
Label protocol Standard Agilent protocol that uses T7 RNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3-labeled CTP (Manual:One-Color Microarray-Based Gene Expression Analysis)
 
Hybridization protocol Standard Agilent protocol using suggested blocking agent, fragmentation buffer and hybridization buffer. Hybridization run for 17 hours at 65°C. (Manual:One-Color Microarray-Based Gene Expression Analysis)
Scan protocol Scanning was performed using Agilnet scanner at 5 uM resolution, green dye channel
Data processing Signal was extracted using feature expression and data were nornalized to the 75th percentile of of all of non-control probes on the microarray using genespring software
 
Submission date Jun 21, 2011
Last update date Sep 02, 2013
Contact name Alessandro Provenzani
E-mail(s) alessandro.provenzani@unitn.it
Phone +390461283094
Organization name University of Trento
Department CIBIO
Lab Laboratory of Genomic Screening
Street address via delle regole 101
City Trento
ZIP/Postal code 38100
Country Italy
 
Platform ID GPL7202
Series (1)
GSE30139 Role of Per2 in the control of liver glycogen metabolism

Data table header descriptions
ID_REF
RAW Raw data
VALUE Normalized data
PMA Present/Marginal/Absent

Data table
ID_REF RAW VALUE PMA
GE_BrightCorner 52452.41 0.16874075 P
DarkCorner 7.3453307 -0.12378454 A
A_52_P616356 6.8215895 -0.5805769 A
A_52_P580582 41.07946 -0.01668644 P
A_52_P403405 13.471699 -0.6141939 A
A_52_P819156 11.705141 -0.70621395 A
A_51_P331831 166.93365 0.019608974 P
A_51_P430630 6.854703 -0.6346922 A
A_52_P502357 6.8613296 -0.24525928 A
A_52_P299964 6.8681135 -0.7748785 A
A_51_P356389 12.51997 -0.09101629 A
A_52_P684402 525.75507 0.024713993 P
A_51_P414208 190.32394 1.4801226 P
A_51_P280918 1015.7824 0.16243029 P
A_52_P613688 15.556841 -1.0301895 A
A_52_P258194 14.005649 -0.73294353 A
A_52_P229271 67.80634 -0.62194276 P
A_52_P214630 96.92858 -0.42779732 P
A_52_P579519 373.3453 0.28278685 P
A_52_P979997 6.963334 -0.5686717 A

Total number of rows: 41252

Table truncated, full table size 1451 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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