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| Status |
Public on Jul 16, 2019 |
| Title |
COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This SuperSeries is composed of the SubSeries listed below
Histone methylation is essential for regulating gene expression during organogenesis to maintain stem cells and execute a proper differentiation program for their descendants. Here, we show that the COMPASS family histone methyltransferase co-factor ASH2L is required for maintaining neural progenitor cells (NPCs) and the production and positioning of projection neurons during neocortex development. Specifically, loss of ASH2L in NPCs results in malformation of the neocortex; the mutant neocortex shows fewer neurons that are also abnormal in composition and laminar position. Moreover, ASH2L loss impairs the trimethylation of H3K4 and the transcriptional machinery specific for Wnt-β-catenin signaling, inhibiting the proliferation ability of NPCs at late stages of neurogenesis by disrupting S phase entry to inhibit cell cycle progression. Overexpressing β-catenin after ASH2L elimination rescues the proliferation deficiency. Therefore, our findings demonstrate ASH2L is crucial for modulating Wnt signaling to maintain NPCs and generate a full complement of neurons during mammalian neocortex development.
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| Overall design |
Refer to individual Series
For RNA-seq, there are two databases and each of within three biological replicates :
1) WT and Ash2l cKO dorsal cortices of E14.5 and 16.5;
2)Primary neurospheres derived from WT and Ash2l cKO dorsal cortices of E14.5, which cultured for 7 days.
For ChIP-seq, we test ASH2L and H3K4me3 binding peak in E14.5 WT dorsal cortices, and H3K4me3 binding peak in E14.5 KO dorsal cortices. ASH2L within two technical replicates.
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| Citation(s) |
31315048 |
| |
| Submission date |
Oct 09, 2018 |
| Last update date |
Oct 18, 2019 |
| Contact name |
Xiaozhong Peng |
| E-mail(s) |
pengxiaozhong@pumc.edu.cn
|
| Phone |
86 69156434
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| Organization name |
Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
|
| Department |
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences
|
| Lab |
Lab of Xiaozhong Peng
|
| Street address |
No.5, Dongdan Santiao, Dongcheng District
|
| City |
Beijing |
| ZIP/Postal code |
100005 |
| Country |
China |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
|
| Samples (23)
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| This SuperSeries is composed of the following SubSeries: |
| GSE120986 |
COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling [RNA-seq] |
| GSE120987 |
COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling [ChIP-seq] |
| GSE133413 |
COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling [RNA-Seq 2] |
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| Relations |
| BioProject |
PRJNA495171 |
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