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Sample GSM3423423 Query DataSets for GSM3423423
Status Public on Jul 16, 2019
Title E14.5_248_6_WT
Sample type SRA
 
Source name dorsal cortex
Organism Mus musculus
Characteristics strain: C57BL/6
age: E14.5
genotype: WT
tissue: dorsal cortex
Extracted molecule total RNA
Extraction protocol RNA was isolated from E14.5 and E16.5 dorsal cortices with TRIzol reagent (Invitrogen) according to the manufacturer’s instructions.The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80°C.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Description E14.5_featureCounts.txt
E14.5_condition.DESeq2_results_allgenes
Data processing Read mapping statistics were obtained from STAR (v2.5.2b)
Fragment assignment statistics obtained from featureCounts in the Subread software packages
Differential gene expression analysis was performed through DESeq2 (v1.14.1)
Genome_build: mm10
Supplementary_files_format_and_content: The output of featureCounts program reports read counts for each gene in each library in a tab-delimited file;The CSV file reports the Gene ID, Gene Symbol with normalized fragment counts for each sample, normalized sum of fragment counts for each group of samples under comparison and the statistics obtained from DESeq2 for each gene.
 
Submission date Oct 09, 2018
Last update date Jul 16, 2019
Contact name Xiaozhong Peng
E-mail(s) pengxiaozhong@pumc.edu.cn
Phone 86 69156434
Organization name Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
Department Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences
Lab Lab of Xiaozhong Peng
Street address No.5, Dongdan Santiao, Dongcheng District
City Beijing
ZIP/Postal code 100005
Country China
 
Platform ID GPL21273
Series (2)
GSE120986 COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling [RNA-seq]
GSE120988 COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling
Relations
BioSample SAMN10221706
SRA SRX4816223

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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