|
| Status |
Public on Jul 16, 2019 |
| Title |
E16.5_376_5_KO |
| Sample type |
SRA |
| |
|
| Source name |
dorsal cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: E16.5
genotype: Ash2l cKO
tissue: dorsal cortex
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from E14.5 and E16.5 dorsal cortices with TRIzol reagent (Invitrogen) according to the manufacturer’s instructions.The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80°C.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
E16.5_featureCounts.txt
E16.5_condition.DESeq2_results_allgenes
|
| Data processing |
Read mapping statistics were obtained from STAR (v2.5.2b)
Fragment assignment statistics obtained from featureCounts in the Subread software packages
Differential gene expression analysis was performed through DESeq2 (v1.14.1)
Genome_build: mm10
Supplementary_files_format_and_content: The output of featureCounts program reports read counts for each gene in each library in a tab-delimited file;The CSV file reports the Gene ID, Gene Symbol with normalized fragment counts for each sample, normalized sum of fragment counts for each group of samples under comparison and the statistics obtained from DESeq2 for each gene.
|
| |
|
| Submission date |
Oct 09, 2018 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Xiaozhong Peng |
| E-mail(s) |
pengxiaozhong@pumc.edu.cn
|
| Phone |
86 69156434
|
| Organization name |
Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
|
| Department |
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences
|
| Lab |
Lab of Xiaozhong Peng
|
| Street address |
No.5, Dongdan Santiao, Dongcheng District
|
| City |
Beijing |
| ZIP/Postal code |
100005 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE120986 |
COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling [RNA-seq] |
| GSE120988 |
COMPASS Family Histone Methyltransferase ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling |
|
| Relations |
| BioSample |
SAMN10221690 |
| SRA |
SRX4816232 |
