GEO DataSets

Analysis of skeletal muscle biopsies collected at 1.5, 4, 10, and 24hr post-meal time from healthy young men subjected to a 24hr fast. Results provide insight into the molecular mechanisms underlying metabolic adaptation to fasting in healthy humans.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 12 individual, 4 time sets

Platform:

GPL10558

Series:

GSE55924

48 Samples

Download data

(Submitter supplied) The aim of the study was to investigate how short-term fasting affects whole-body energy homeostasis and skeletal muscle energy/nutrient-sensing pathways and transcriptome in humans. For this purpose, twelve young healthy men were studied during a 24-hour fast. Skeletal muscle biopsies were collected and analyzed at baseline and after 4, 10 and 24h of fasting. As expected, fasting induced a time-dependent decrease in plasma insulin and leptin levels, whereas levels of ketone bodies and free fatty acids increased. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5473

Platform:

GPL10558

48 Samples

Download data: TXT

(Submitter supplied) The adaptive mechanisms in response to excess energy supply are still poorly known in humans. Our aims were to define metabolic responses and changes in gene expression in skeletal muscle of healthy volunteers during fat overfeeding.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) Forkhead box class O (FoxO) transcription factors regulate whole body energy metabolism, skeletal muscle mass and substrate switching. To elucidate the role of FOXO in skeletal muscle, dominant negative (dn) constructs for FOXO1 (FOXO1dn) or FOXO3 (FOXO3dn) were transfected by electroporation into mouse tibialis anterior muscle and glucose uptake, signal transduction, and glucose stimulated gene expression profiles were assessed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17400

24 Samples

Download data: CEL

(Submitter supplied) Analysis of Class II Histone Deacetylase (HDAC) regulation of hepatic gluconeogenesis at the gene expression level. We show that in liver, Class IIa HDACs (HDAC4, 5, and 7) are all phosphorylated and excluded from the nucleus by AMPK family kinases. In response to the fasting hormone glucagon, Class IIa HDACs rapidly translocate to the nucleus where they directly bind to the promoters of gluconeogenic enzymes such as G6Pase. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6103

16 Samples

Download data: TXT

(Submitter supplied) We performed the circadian transcriptome analysis using the skeletal muscle from sedentary and exercised mice either in the early rest phase (ZT3) or in the early active phase (ZT15). By the combination with circadian transcriptomic and metabolomic analysis, we revealed time-of-day-dependent remodeling of circadian muscular metabolic pathways involved in glucose and glycerol metabolism after exercise. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

140 Samples

Download data: TXT

(Submitter supplied) Prolonged fasting-induced changes in rat tibialis anterior muscle transcriptome Skeletal muscle is of primary importance for metabolism, thermogenesis and locomotion. However, muscle integrity is inevitably challenged throughout life, and muscle atrophy occurs in various situations, being associated with development of metabolic diseases. There is virtually no fully effective countermeasure today to fight the loss of muscle mass. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

9 Samples

Download data: CEL, CHP

(Submitter supplied) Circadian misalignment, such as in shift work, has been associated with obesity and type 2 diabetes, however, direct effects of circadian misalignment on skeletal muscle insulin sensitivity and muscle molecular circadian clock have never been investigated in humans. Here we investigated insulin sensitivity and muscle metabolism in fourteen healthy young lean men (age 22.4 ± 2.8 years; BMI 22.3 ± 2.1 kg/m2 [mean ± SD]) after a 3-day control protocol and a 3.5-day misalignment protocol induced by a 12-h rapid shift of the behavioral cycle. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

47 Samples

Download data: CEL

(Submitter supplied) The circadian regulation of transcriptional processes has a broad impact on cell metabolism. Here, we compared the diurnal transcriptome of human skeletal muscle conducted on serial muscle biopsies in vivo with profiles of human skeletal myotubes synchronized in vitro. Extensive rhythmic transcription was observed in human skeletal muscle in comparison to in vitro cell culture. However, nearly half of the in vivo rhythmicity was lost at the mRNA accumulation level. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

100 Samples

Download data: TXT

(Submitter supplied) The circadian regulation of transcriptional processes has a broad impact on cell metabolism. Here, we compared the diurnal transcriptome of human skeletal muscle conducted on serial muscle biopsies in vivo with profiles of human skeletal myotubes synchronized in vitro. Extensive rhythmic transcription was observed in human skeletal muscle in comparison to in vitro cell culture. However, nearly half of the in vivo rhythmicity was lost at the mRNA accumulation level. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

57 Samples

Download data: TXT

(Submitter supplied) We assessed vastus lateralis muscle gene expression levels of 12 women with the metabolic syndrome before and after a 6 month exercise training program

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4909

Platform:

GPL6244

24 Samples

Download data: CEL

Analysis of skeletal muscle from metabolic syndrome (MS) female patients before and after 6 months of exercise training. MS is associated with insulin resistance. Results provide insight into the molecular mechanisms underlying insulin-resistant skeletal muscle in MS patients.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state, 12 individual, 2 protocol sets

Platform:

GPL6244

Series:

GSE43760

24 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

1531 Samples

Download data: CEL

(Submitter supplied) The aim of this work was to produce a molecular signature of human muscle responses to supervised resistance training and examine how such a profile relates to established aerobic exercise adaptation gene networks.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

89 Samples

Download data: CEL

(Submitter supplied) The overall objective of the heritage project is to study the role of the genotype in cardiovascular,metabolic and hormonal responses to aerobic exercise training and the contribution of regular exercise to changes in several cardiovascular disease and diabetes risk factors. PLEASE NOTE THE POST-TRAINING GENE CHIP FILES HAVE NEVER BEEN RELEASED ON GEO. PLEASE ALSO NOTE THAT DUE TO THE OUTDATED INSULIN ASSAY UTILISED IN THE HERITAGE STUDY, THE INSULIN DATA WAS NOT COMPARABLE WITH ANY MORE RECENT MODERN STUDIES.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

52 Samples

Download data: CEL

(Submitter supplied) Primary mitochondrial respiratory chain (RC) diseases are heterogeneous in etiology and manifestations but collectively impair cellular energy metabolism. To identify a common cellular response to RC disease, systems biology level transcriptome investigations were performed in human RC disease skeletal muscle and fibroblasts. Global transcriptional and post-transcriptional dysregulation in a tissue-specific fashion was identified across diverse RC complex and genetic etiologies. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16240

53 Samples

Download data: CEL

(Submitter supplied) Fasting is the process of metabolic adaption to food deprivation that is taking place in most organisms, e.g. during the daily resting phase in mammals. Furthermore, in biomedical research fasting is used in most metabolic studies to synchronize nutritional states of study subjects. Because there is a lack of standardization for this procedure, we need a deeper understanding of the dynamics and the molecular players in fasting. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4918

Platform:

GPL11533

30 Samples

Download data: CEL

Analysis of white adipose tissue, liver and skeletal muscle in 24h fasted C57Bl/6J males. Results provide insight into the molecular mechanisms underlying metabolic adaption to food deprivation in various tissues.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 protocol, 3 tissue sets

Platform:

GPL11533

Series:

GSE46495

30 Samples

Download data: CEL

(Submitter supplied) The effects of fasting have been studied extensively, predominantly on isolated processes, within a specific organ. No comprehensive study of the adaptations was available for different organs, let alone interrelating them, which left the understanding of the body’s orchestration of fasting response limited. The gene expression profiles of brain, small intestine, kidney, liver and skeletal muscle were therefore studied in mice subjected to short, moderate and prolonged fasting. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3893

Platform:

GPL11012

122 Samples

Download data: TXT

Analysis of brain, small intestine, kidney, liver, and skeletal muscle subjected to 0-72 hrs of fasting. Adapting to starvation requires an interorgan integration of metabolic activity to protect vital organs. Results provide insight into molecular mechanisms underlying this adaptive response.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 5 time, 5 tissue sets

Platform:

GPL11012

Series:

GSE24504

122 Samples

Download data: TXT