GEO DataSets

(Submitter supplied) DLX3 is a homeodomain transcription factor involved in epidermal differentiation. Here we investigated the distribution of DLX3 DNA binding sites in suprabasal differentiating keratinocytes using ChIP-seq.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

3 Samples

Download data: BED, BEDGRAPH

(Submitter supplied) DLX3 is a homeodomain transcription factor involved in epidermal and hair follicle differentiation. Epidermal specific deletion of DLX3 leads to IL17 related inflammation. However, the downstream signals from DLX3-deficient keratinocytes that initiates IL17 associated inflammation is not well-characterized. Therefore we performed acute Dlx3 deletion to identify the initial molecular changes causing the skin inflammatory reponse and tried to rescue the immune phenotype by STAT3 inhibitor treatment (Cryptotanshinone). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17021 GPL21493

29 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL16791 GPL571

11 Samples

Download data: CEL

(Submitter supplied) Reinstatement of DLX3 into SCC13 cells upregulates genes involved with cell cycle exit, signaling, and adhesion whiles downregulates genes involved with cell death, proliferation, and movement.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

8 Samples

Download data: XLS

(Submitter supplied) DLX3 is expressed by differentiated cells in human skin and it has a functional role in epidermal maturation. We used microarray data analysis to assess gene expression in keratinocytes as consequence of DLX3 expression. We identified a specific subset of genes which are involved in cell cycle regulation and modulate the differentiation program in epidermal cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

3 Samples

Download data: CEL

(Submitter supplied) Emerging evidence suggests that homeoproteins can act as modulators of tumor initiation and progression. Our findings show that loss of DLX3 tumor suppressive function acts as a tumor promoter through regulation of ERBB2 and EGFR signaling and also support an important inhibitory role of DLX3 in skin cancer promotion and progression, that may potentially be used as a promising prognostic marker.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24247 GPL21493

11 Samples

Download data: XLSX

(Submitter supplied) We analysed the genexpression of dental follicle cells (DFCs) after 3 days osteogenic differentiation with BMP2 after transfection with a DLX3 plasmid (pDLX3) and after transfection with an empty plasmid (pEV)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11532

4 Samples

Download data: CEL

(Submitter supplied) DLX4 is a member of DLX family of mammalian homeobox genes and cis linked sister gene of Dlx3. Misexpression of Dlx4 is associated with tumorigenicity. However,Dlx4 role in epidermal development and homeostasis is not characterized. In this study, we compared Wild type (DLX4-WT) and DLX4 deficient (DLX4-KO) Skin transcriptomes.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

10 Samples

Download data: XLSX

(Submitter supplied) The AhR is a ligand activated transcription factor that may be important in normal skin physiology. We compared gene expression profiles between AhR Wt and AhR KO primary mouse keratinocyte cultures. We identified 391 genes that were differentially expressed with a 1.5 fold cutoff and p<.05, and identified the AhR as an important regulator of genes involved in normal epidermal differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

7 Samples

Download data: CEL

(Submitter supplied) Disrupted skin barrier due to altered keratinocyte differentiation is common in pathologic conditions such as atopic dermatitis, ichthyosis and psoriasis. However, the molecular cascades governing keratinocyte terminal differentiation are still poorly understood. We have previously demonstrated that a dominant mutation in ZNF750 leads to a clinical phenotype that reminiscent of psoriasis and seborrheic dermatitis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4599

Platform:

GPL6244

6 Samples

Download data: CEL

Analysis of ZNF750-silenced HaCaT keratinocytes (KCs) at day 12 of calcium-induced differentiation. ZNF750 knockdown in HaCaT KCs leads to sustained cell proliferation and decreased apoptosis. Results provide insight into molecular mechanisms underlying keratinocyte terminal differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL6244

Series:

GSE38039

6 Samples

Download data: CEL

(Submitter supplied) In an RNAseq analysis, we have identified the HOXC13-AS significantly downregulated in wound biopsies and epidermal cells compared to skin counterparts. To study the genes regulated by HOXC13-AS, we transfected HOXC13-AS siRNA pool into human primary epidermal keratinocytes to knockdown HOXC13-AS and induced cell differentiation for 3 days by 1.5 mM calcium. We performed a global transcriptome analysis of keratinocytes upon the HOXC13-AS knockdown using Affymetrix arrays.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL24324

6 Samples

Download data: CEL

(Submitter supplied) Due to microRNAs' (miRs) important functions and high potential for disease diagnosis and therapy, increasing efforts have been put to understand their role in wound repair and identify targetable miRs for wound treatment. However, lack of knowledge about miR-mediated gene expression in human wound tissues hinders the recognition of clinically relevant miRs. Here we profiled genome-wide miR and mRNA expression by RNA-sequencing in the same set of samples from human normal acute wounds and chronic non-healing venous ulcers (VU). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL16791 GPL20301

40 Samples

Download data: TXT

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived femoral diaphysis and metaphysis transcriptome profiling (RNA-seq) to determine pathways and networks dependent on Dlx3 during bone development and homeostasis. Methods: mRNA profiles of diaphysis and metaphysis isolated from the femur of 5-week-old wild-type (WT) and Dlx3Oc-cKO (OC-cre;Dlx3f/-) conditional knockout mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: TXT

(Submitter supplied) We analysed gene expression profiles in dental follicle cells after 48 hours of overexpression with the transcription factor DLX3.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11532

4 Samples

Download data: CEL

(Submitter supplied) Comparison between pattern of gene expression in uncultured keratinocytes derived from the epidermis of embryonic mice (E15.5) versus newborn mice and comparison between pattern of gene expression in primary keratinocytes derived from newborn mice plus/minus activated Notch1 expression. Keywords = mouse Keywords = keratinocyte Keywords = skin Keywords = embryonic development. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS790

Platform:

GPL81

4 Samples

Download data

Comparison of gene expression in keratinocytes from embryos at 15.5 days of gestation to that from 2 day newborns infected with an adenovirus expressing a constitutively activate Notch1. Results indicate that Notch1 promotes differentiation in part through caspase 3.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 development stage, 3 infection sets

Platform:

GPL81

Series:

GSE1569

4 Samples

Download data

(Submitter supplied) This study investigates the role of ADAM17 (a disintegrin and metalloproteinase 17) in skin homeostasis. Here, we show that mice lacking ADAM17 in keratinocytes have a normal epidermal barrier and skin architecture at birth, but develop pronounced defects in epidermal barrier integrity soon after birth and chronic dermatitis as adults. The dysregulated expression of epidermal differentiation proteins becomes evident 2 days after birth, followed by transepidermal water loss and inflammatory immune cell infiltration. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

4 Samples

Download data: TXT

(Submitter supplied) This transcriptome study is intended to discover the effects of MYC and TP63 on human keratinocytes differentiation at the genome level.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4803

48 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

20 Samples

Download data: CEL, CHP