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| Status |
Public on Jun 15, 2022 |
| Title |
Human skin specific long noncoding RNA HOXC13-AS regulates keratinocyte differentiation by inferfering with Golgi-ER retrograde transport |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In an RNAseq analysis, we have identified the HOXC13-AS significantly downregulated in wound biopsies and epidermal cells compared to skin counterparts.
To study the genes regulated by HOXC13-AS, we transfected HOXC13-AS siRNA pool into human primary epidermal keratinocytes to knockdown HOXC13-AS and induced cell differentiation for 3 days by 1.5 mM calcium. We performed a global transcriptome analysis of keratinocytes upon the HOXC13-AS knockdown using Affymetrix arrays.
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| Overall design |
Gene expression profiling of differentiated keratinocytes transfected with either siRNA control (n = 3) or si-HOXC13-AS (n = 3) was carried out by using human Clariom™ S assay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA) core facility at Karolinska Institutet.
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| Contributor(s) |
Landén NX, Zhang L |
| Citation(s) |
36869179 |
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| Submission date |
Jun 14, 2022 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Ning Xu |
| Organization name |
Karolinska Institutet
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| Street address |
CMM L8:02
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| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
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| Platforms (1) |
| GPL24324 |
[Clariom_S_Human_HT] Affymetrix Clariom S Assay HT, Human (Includes Pico Assay) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA849206 |
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