GEO DataSets

(Submitter supplied) Macrophages were infected with low (PR8) and high pathogenic influenza viruses (FPV and H5N1). To our surprise a genome-wide comparative systems biology approach revealed that in contrast PR8 infections with HPAIV H5N1 and FPV result in a reduced immune response of human macrophages contradicting a primary role of this cell type for the cytokine storm. Our data point to a viral strategy of HPAIV to bypass a major amplifier of the initial local inflammatory response thereby hampering antiviral effector mechanisms and facilitating virus spreading and systemic disease.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

12 Samples

Download data: CEL, CHP

(Submitter supplied) Human disease caused by highly pathogenic avian influenza (HPAI) H5N1 can lead to a rapidly progressive viral pneumonia leading to acute respiratory distress syndrome. There is increasing evidence suggests a role for virus-induced cytokine dysregulation in contributing to the pathogenesis of human H5N1 disease. The key target cells for the virus in the lung are the alveolar epithelium and alveolar macrophages, and previous data has shown that compared to seasonal human influenza viruses, equivalent infecting doses of H5N1 viruses markedly up-regulate pro-inflammatory cytokines in both primary cell types in vitro. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3595

Platform:

GPL6244

27 Samples

Download data: CEL, CHP

Analysis of macrophages at 1, 3, and 6 hours post-infection with H1N1 or H5N1 viruses in vitro. The avian H5N1 virus is highly pathogenic, while the swine H1N1 virus is less so. Alveolar macrophages are targets of H5N1. Results provide insight into the host response to H1N1 and H5N1 infections.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 individual, 3 infection, 3 time sets

Platform:

GPL6244

Series:

GSE18816

27 Samples

Download data: CEL, CHP

(Submitter supplied) Modulating the host response is a promising approach to treating influenza, a virus whose pathogenesis is determined in part by the host response it elicits. Though the pathogenicity of emerging H7N9 influenza virus has been reported in several animal models, these studies have not included a detailed characterization of the host response following infection. To this end, we characterized the transcriptomic response of BALB/c mice infected with H7N9 (A/Anhui/1/2013) virus and compared it to the responses induced by H5N1 (A/Vietnam/1203/2004), H7N7 (A/Netherlands/219/2003) or H1N1 (A/Mexico/4482/2009) viruses. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

68 Samples

Download data: TXT

(Submitter supplied) While pandemic 2009 H1N1 influenza A viruses were responsible for numerous severe infections in humans, these viruses do not typically cause corresponding severe disease in mammalian models. However, the generation of a virulent 2009 H1N1 virus following serial lung passage in mice has allowed for the modeling of human lung pathology in this species. Genetic determinants of mouse-adapted 2009 H1N1 viral pathogenicity have been identified, but the molecular and signaling characteristics of the host response following infection with this adapted virus have not been described. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

64 Samples

Download data: TXT

(Submitter supplied) The goals of this study are to compare NGS-derived whole transcriptome profiles (RNA-seq) of H5N1 infected A549 cells overexpressing either negative control mimic or miR-324-5p mimic

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

3 Samples

Download data: TXT

(Submitter supplied) Background. The pathogenesis of influenza A virus subtype H5N1 (hearafter, "H5N1") infection in humans is not completely understood, although hypercytokinemia is thought to play a role. We previously reported that most H5N1 viruses induce high cytokine responses in human macrophages, whereas some H5N1 viruses induce only a low level of cytokine production similar to that induced by seasonal viruses. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13607

12 Samples

Download data: TXT

(Submitter supplied) Highly pathogenic avian influenza viruses (HPAIV) induce severe inflammation in poultry and men. There is still an ongoing threat that these viruses may acquire the capability to freely spread as novel pandemic virus strains that may cause major morbidity and mortality. One characteristic of HPAIV infections is the induction of a cytokine burst that strongly contributes to viral pathogenicity. It has been suggested, that this cytokine overexpression is an intrinsic feature of infected cells and involves hyperinduction of p38 mitogen activated protein kinase (MAPK). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

9 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

18 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL9115

18 Samples

Download data: TXT

(Submitter supplied) For the small RNA portion in the whole transcriptome, a total of 105,842,096 filtered high-quality sequencing reads, representing 2,854,995 unique miRNA species, were generated for miRNAs for H1N1- or H5N1- infected macrophage cell populations at 1-, 3-, and 6-hr post-infection. A total of 361 mature miRNAs and 113 mature star miRNAs were identified from the samples. We calculated the miRNA expression fold change in influenza A viruses infected cells compared to mock-infected control cells andassessed the significance of the differential expression for each miRNA using Z-statistics. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9115

9 Samples

Download data: XLS

(Submitter supplied) We generated an average of 868 million nucleotides and 30 folds coverage per sample. More than 76% of the sequencing reads were mapped to the human genome, and 45.5% of the annotated reference genes were mapped by at least 10 reads. Differential expression quantification showed that H5N1 virus elicits greater changes in host gene expression than the H1N1 virus at nearly all three time points with 1198,273,and 620 up-regulated in H5N1, and 426,285, and 523 in H1N1 compared with mock control (fold-change >= 2.0, and p-value <= 0.05). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9115

9 Samples

Download data: TXT, XLS

(Submitter supplied) This study revealed important similarities but also critical differences between the H5N1 and 1918-reassortant viruses, highlighting aspects of the host–pathogen interface caused by highly virulent influenza viruses.

Organism:

Macaca mulatta; Macaca fascicularis

Type:

Expression profiling by array

Platform:

GPL9861

72 Samples

Download data: TXT

(Submitter supplied) Highly pathogenic influenza virus inhibit Inflammatory Responses in Monocytes via Activation of the Rar-Related Orphan Receptor Alpha (RORalpha). Low (PR8) and high pathogenic influenza viruses (FPV and H5N1) were used.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL, CHP

(Submitter supplied) The highly pathogenic avian influenza (HPAI) H5N1 viruses continue to circulate in nature and threaten public health. Although several viral determinants and host factors that influence the virulence of HPAI H5N1 viruses in mammals have been identified, the detailed molecular mechanism remains poorly defined and requires further clarification. In our previous studies, we characterized two naturally isolated HPAI H5N1 viruses that had similar viral genomes but differed substantially in their lethality in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

9 Samples

Download data: TXT

(Submitter supplied) As a mild, highly contagious, respiratory disease, swine influenza always damages the innate immune systems, and increases susceptibility to secondary infections which results in considerable morbidity and mortality in pigs. Nevertheless, the systematical host response of pigs to swine influenza virus infection remains largely unknown. To explore these, a time-course gene expression profiling was performed to detect comprehensive analysis of the global host response induced by H1N1 swine influenza virus in pigs.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL10162

12 Samples

Download data: TXT

(Submitter supplied) Array analysis of total lung RNA obtained from mice 12,16,24 h post infection with influenza. Strains used were Mock, PR8, X31, VN62 (1x10^5pfu)

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

30 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL15271 GPL6947

133 Samples

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