 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 01, 2011 |
| Title |
Early and sustained innate immune response defines pathology and death in nonhuman primates infected by highly pathogenic influenza virus. |
| Platform organism |
Macaca mulatta |
| Sample organism |
Macaca fascicularis |
| Experiment type |
Expression profiling by array
|
| Summary |
This study revealed important similarities but also critical differences between the H5N1 and 1918-reassortant viruses, highlighting aspects of the host–pathogen interface caused by highly virulent influenza viruses.
|
| |
|
| Overall design |
Animals assigned to 4 experimental groups (n = 8) matched for age, weight, and sex, were inoculated by intratracheal, intranasal, tonsillar, and conjunctival routes with a total of 107 pfu of A/Vietnam/1203/2004 (H5N1) virus, A/Texas/36/91 (H1N1) virus or reassortants of this virus containing either 2 (HA, NA) or 3 (HA, NA, NS) genes from the 1918 virus. Two animals per group were scheduled for sacrifice on days 1, 2, 4, and 7. Two additional animals were used as uninfected control animals and killed on day 7. Oligoarray analyses consisted of comparing array profiles of individual lung samples (with representative pathology and degree of infection) from infected animals to pooled equal masses of mRNA from all lung lobes of 7 reference cynomolgus macaques obtained through the tissue program of the University of Washington National Primate Research Center. There are 2 technical array replicates fro each sample.
|
| |
|
| Contributor(s) |
Baskin CR, Bielefeldt-Ohman H, Tumpey TM, Sabourin PJ, Katze M, Long JP, García-Sastre A, Tolnay A, Albrecht R, Pyles oA, Olson PH, Aicher LD, Rosenzweig ER, Murali-Krishna K, Clark EA, Kotur MS, Fornek JL, Proll S, Palermo RE, Sabourin CL |
| Citation(s) |
19218453 |
| |
| Submission date |
Oct 31, 2011 |
| Last update date |
Feb 04, 2013 |
| Contact name |
Michael Katze |
| E-mail(s) |
data@viromics.washington.edu
|
| Organization name |
University of Washington
|
| Department |
Microbiology
|
| Lab |
Michael G. Katze, Ph.D
|
| Street address |
Rosen Building 960 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9861 |
Agilent-015421 Rhesus Macaque Gene Expression Microarray (Feature Number version) |
|
| Samples (72)
|
|
| Relations |
| BioProject |
PRJNA148857 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33351_RAW.tar |
477.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...