GEO DataSets

(Submitter supplied) Targets of Retinoic Acid (RA) were identified in primary human epidermal keratinocytes grown in the presence or absence of all-trans retinoic acid for 1, 4, 24, 48 and 72 hours. Targets of Thyroid Hormone (T3) were identified in primary human epidermal keratinocytes grown in the presence or absence of the hormone; same controls as for RA.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

15 Samples

Download data: CEL, CHP

(Submitter supplied) Targets of Retinoic Acid (RA) and 3,4-didehydroretinoic acid (ddRA) were identified in primary human epidermal keratinocytes grown in the presence of atRA or ddRA for 4 and 24 hours. Retinoids (natural forms and synthetic derivatives of vitamin A) are used as therapeutic agents for numerous skin diseases such as keratinization disorders (e.g. ichthyoses) and psoriasis. Two endogenous ligands for retinoic acid receptors exist, retinoic acid (atRA) and 3,4-didehydroretinoic acid (ddRA). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

36 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Glucocorticoids (GCs) have a long history of use as therapeutic agents for numerous skin diseases. Surprisingly, their specific molecular effects are largely unknown. To characterize GC action in epidermis, we compared the transcriptional profiles of primary human keratinocytes untreated and treated with dexamethasone (DEX) for 1, 4, 24, 48 and 72 hours using large-scale microarray analyses. The majority of genes were found regulated only after 24 hours and remained regulated throughout the treatment. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5208

Platform:

GPL8300

20 Samples

Download data: CEL

Analysis of cultured epidermal keratinocytes treated with the synthetic glucocorticoid (GC) dexamethasone for up to 72 hours. GCs are used as therapeutic agents for skin conditions. Results provide insight into the molecular response of the epidermis to GCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 5 time sets

Platform:

GPL8300

Series:

GSE26487

20 Samples

Download data: CEL

(Submitter supplied) In inflamed tissue, normal signal transduction pathways are altered by extracellular signals. For example, the JNK pathway is activated in psoriatic skin, which makes it an attractive target for treatment. To define comprehensively the JNK-regulated genes in human epidermal keratinocytes, we compared the transcriptional profiles of control and JNK inhibitor-treated keratinocytes, using DNA microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2081

Platform:

GPL8300

8 Samples

Download data

Analysis of keratinocytes at various time points up to 48 hours following treatment with the Jun kinase (JNK) inhibitor SP600125. SP600125 is a reversible ATP-competitive inhibitor specific for the three isoforms of JNK. Results identify JNK-regulated genes in epidermal keratinocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL8300

Series:

GSE4828

8 Samples

Download data

(Submitter supplied) Interleukin-1 is a proinflammatory and immunomodulatory cytokine that plays a crucial role in inflammatory diseases of the skin, including bacterial infections, bullous diseases, UV damage and especially psoriasis. To characterize the molecular effects of IL-1 in epidermis, we defined the transcriptional changes in human epidermal keratinocytes 1, 4, 24, and 48 h after treatment with IL-1a. IL-1 significantly regulated 388 genes, including genes associated with proteolysis, adhesion, signal transduction, proliferation, and epidermal differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3011

Platform:

GPL571

8 Samples

Download data: CEL, CHP

Analysis of epidermal keratinocytes at various time points up to 48 hours post-interleukin-1 (IL-1) treatment. IL-1 is a proinflammatory and immunomodulatory cytokine that plays a role in inflammatory diseases of the skin. Results provide insight into gene products that mediate the effects of IL-1.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL571

Series:

GSE9120

8 Samples

Download data: CEL, CHP

(Submitter supplied) Whole genome expression array comparison of primary keratinocytes to retinoic acid-differentiated hES cell derivatives Cells were differentiated according to Metallo et al. Stem Cells 2008

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8906

6 Samples

Download data: PAIR

(Submitter supplied) Cultured epidermal keratinocyte controls used for IFNg, TNFa and IL1 treatment. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1846

Platform:

GPL8300

9 Samples

Download data: CEL, EXP

Analysis of keratinocytes treated with interferon gamma (IFN-gamma) for up to 48 hours. IFN-gamma is a multifunctional, immunomodulatory cytokine with cell type-specific antiviral activities. Results provide insight into molecular processes regulated by IFN-gamma in keratinocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL8300

Series:

GSE440

9 Samples

Download data: CEL, EXP

(Submitter supplied) Cultured keratinocytes treated with TNFa in the presence or absence of NFkB inhibitor; time course 1, 4, 24 & 48 hrs. Keywords = Epidermis Keywords = skin Keywords = keratinocytes Keywords = inflammation Keywords = NFkB Keywords = TNFa Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1289

Platform:

GPL8300

16 Samples

Download data: CEL, EXP

Analysis of epidermal keratinocytes treated with TNFalpha in the presence of 10 uM parthenolide, an NF-kappa B inhibitor. Cells examined at various time points up to 48 hours following TNFalpha treatment. Results identify NF-kappa B-dependent subset of TNFalpha-regulated genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 time sets

Platform:

GPL8300

Series:

GSE2489

16 Samples

Download data: CEL, EXP

(Submitter supplied) Our goal was to demonstrate the similarity between the original keratinocytes and iPSC-derived keratinocytes from the same individual Profiles of original keratinocytes, iPSCs, IPSC-derived keratinocytes, iPSC-derived fibroblasts from a single individual and normal fibroblasts from a second individual were compared

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

8 Samples

Download data: CEL

(Submitter supplied) Disrupted skin barrier due to altered keratinocyte differentiation is common in pathologic conditions such as atopic dermatitis, ichthyosis and psoriasis. However, the molecular cascades governing keratinocyte terminal differentiation are still poorly understood. We have previously demonstrated that a dominant mutation in ZNF750 leads to a clinical phenotype that reminiscent of psoriasis and seborrheic dermatitis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4599

Platform:

GPL6244

6 Samples

Download data: CEL

Analysis of ZNF750-silenced HaCaT keratinocytes (KCs) at day 12 of calcium-induced differentiation. ZNF750 knockdown in HaCaT KCs leads to sustained cell proliferation and decreased apoptosis. Results provide insight into molecular mechanisms underlying keratinocyte terminal differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL6244

Series:

GSE38039

6 Samples

Download data: CEL

(Submitter supplied) Epidermis, a continuously self-renewing and differentiating organ, produces a protective stratum corneum that shields us from external chemical, physical and microbial threats. Epidermal differentiation is a multi-step process regulated by influences, some unknown, others insufficiently explored. Detachment of keratinocytes from the basement membrane is one such pro-differentiation stimulus. Here, we define the transcriptional changes during differentiation, especially those caused by detachment from the substratum. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

2 Samples

Download data: CEL

(Submitter supplied) To identify putative target genes of the transcription factor Klf9 in primary keratinocytes we over-expressed Klf9 in these cells using a lentiviral delivery system. We detected several hundred genes that show differential expression levels following ectopic Klf9 expression including several genes that are involved in proliferation and cell cycle control. These results allow insights into the mechanisms by which Klf9 regulates proliferation in primary keratinocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5177

Platform:

GPL6480

6 Samples

Download data: TXT

(Submitter supplied) To detect time of day dependent gene expression in human epidermis suction blister samples from 20 healthy subjects were obtained at three different time points throughout the day. RNA from 20 subjects were used to perform whole genome microarray analysis. Microarrays from 19 subjects showed sufficient quality to perform analysis for differential gene expression. We detected significant differential expression levels for several canonical clock genes such as Per1, Per2, Per3, Bmal1 and Rev-Erb_alpha throughout the day. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5176

Platform:

GPL6480

57 Samples

Download data: TXT

Analysis of primary neonatal foreskin keratinocytes overexpressing Kruppel-like factor 9 (KLF9) for up to 7 days. KLF9 is a transcription factor that binds to GC box elements. Results identify downstream targets of KLF9.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol, 3 time sets

Platform:

GPL6480

Series:

GSE36014

6 Samples

Download data: TXT