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Series GSE9120 Query DataSets for GSE9120
Status Public on Sep 22, 2007
Title IL-1 treatment of human epidermal keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Interleukin-1 is a proinflammatory and immunomodulatory cytokine that plays a crucial role in inflammatory diseases of the skin, including bacterial infections, bullous diseases, UV damage and especially psoriasis. To characterize the molecular effects of IL-1 in epidermis, we defined the transcriptional changes in human epidermal keratinocytes 1, 4, 24, and 48 h after treatment with IL-1a. IL-1 significantly regulated 388 genes, including genes associated with proteolysis, adhesion, signal transduction, proliferation, and epidermal differentiation. IL-1 induces many genes that have antimicrobial function. Secreted cytokines, chemokines, growth factors, and their receptors are the prominent targets of IL-1 regulation, including IL-8, IL-19, elafin, C3, and S100A proteins, which implicates IL-1 in the pathogenesis of inflammatory diseases. IL-1 induced not only proliferation-associated genes but also differentiation marker genes such as transglutaminase-1 and involucrin, which suggests that IL-1 plays an important role in the aberrant proliferation and differentiation seen in psoriasis. Correlation of IL-1 regulated genes with the TNFa and IFNg regulated ones showed more similarities between IL-1 and TNFa than IL-1 and IFNg, whereas Oncostatin-M affected a largely unrelated set of genes. IL-1 regulates many genes previously shown to be specifically over-expressed in psoriasis. In summary, IL-1 regulates a characteristic set of genes that define its specific contribution to inflammation and aberrant differentiation in skin diseases.
Keywords: Time course
Overall design keratinocytes were treated with 25 ng/ml IL-1 and transcriptomes compared to untreated controls at 1, 4, 24 and 48 h post treatment.
Contributor(s) Blumenberg M, Yano S, Banno T, Walsh R
Citation(s) 17941080
Submission date Sep 20, 2007
Last update date Dec 06, 2018
Contact name Miroslav Blumenberg
Phone 212 263-5924
Organization name NYU School of Medicine
Department Dermatology
Lab Blumenberg
Street address 455 First Ave #874
City New York
State/province NY
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM230879 Human epidermal keratinocytes treated with IL-1 1hr
GSM230880 Human epidermal keratinocytes controls 1 hr
GSM230881 Human epidermal keratinocytes control 4 hr
BioProject PRJNA102655

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Supplementary file Size Download File type/resource
GSE9120_RAW.tar 16.1 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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