GEO DataSets

(Submitter supplied) Administration of attenuated autologous Theileria parva infected cells can be used as an alternative to the infection-and-treatment method for inducing immunological protection against East Coast Fever. The mechanism of attenuation however has not been described. Using RNA sequencing, the transcriptomes of both host and parasite in uninfected (control), pathogenic (day 7 post-infection) and attenuated (day 69 post-infection) T. more...

Organism:

Theileria parva; Bos taurus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32030 GPL33841

16 Samples

Download data: CSV

(Submitter supplied) To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites

Organism:

Babesia bigemina

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32692

6 Samples

Download data: XLSX

(Submitter supplied) Babesia parasites transition between a mammalian host, where they cause babesiosis, and the tick vector that transmits them. This transition provides an environmental signal resulting in altered gene expression allowing the completion of the parasite’s life cycle. A comparison of the different life stages that occur within mammalian and tick hosts can provide insight into the adaptation of Babesia to these different environments. more...

Organism:

Babesia bovis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28027

6 Samples

Download data: TXT

(Submitter supplied) Using Illumina technology, we aim to identify global change in gene expression of bovine leucocytes and Theileria transcrits upon curcumin treatment

Organism:

Theileria annulata; Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25502

2 Samples

Download data: TXT

(Submitter supplied) Investigation of parasite (T. annulata) gene expression over the course of the life-cycle (sporozoite->macroschizont->merozoite->piroplasm). The study focused on the expression of known and putative transcription factors, in particular members of the ApiAP2 gene family. Up-stream motifs associated with stage-specifically expressed genes were identified during the course of the analysis.

Organism:

Theileria annulata; Bos taurus

Type:

Expression profiling by array

Platform:

GPL20733

20 Samples

Download data: TXT

(Submitter supplied) Human babesiosis, especially caused by the cattle derived Babesia divergens parasite, is on the increase, resulting in renewed attentiveness to this potentially life threatening emerging zoonotic disease. The molecular mechanisms underlying the pathophysiology and intra-erythrocytic development of these parasites are poorly understood. This impedes concerted efforts aimed at the discovery of novel anti-babesiacidal agents. more...

Organism:

Babesia bovis; Babesia divergens

Type:

Expression profiling by array

Platform:

GPL20008

6 Samples

Download data: GPR

(Submitter supplied) Geographical distinct virulent Babesia bovis strains have similar gene expression changes as they go through attenuation.

Organism:

Babesia bovis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17815

6 Samples

Download data: TXT

(Submitter supplied) The experiment investigates bovine gene expression in response to BW720c treatment in uninfected and Theileria annulata-infected cell cultures Theileria annulata, an intracellular parasite of bovine lymphoid cells, induces substantial phenotypic alterations to its host cell including continuous proliferation, cytoskeletal changes and resistance to apoptosis. While parasite induced modulation of host cell signal transduction pathways and NFκB activation are established, there remains considerable speculation on the complexities of the parasite directed control mechanisms that govern these radical changes to the host cell. more...

Organism:

Theileria annulata; Bos taurus

Type:

Expression profiling by array

Platform:

GPL16696

18 Samples

Download data: TXT

(Submitter supplied) The experiment investigates bovine gene expression in response to LPS in uninfected and Theileria annulata-infected cell cultures A subset of genes are identified which are activated in response to LPS stimulation with further modulation due to parasite infection.

Organism:

Theileria annulata; Bos taurus

Type:

Expression profiling by array

Platform:

GPL15339

18 Samples

Download data: TXT

(Submitter supplied) The protozoan parasite Theileria parva infects and transforms bovine lymphocytes inducing uncontrolled proliferation. The transforming schizont resides free in the host cell cytoplasm and it is assumed that proteins released from the parasite contribute to host cell transformation and parasite persistence. The identification and characterisation of parasite genes encoding candidate secreted proteins constitutes a first step towards elucidating this complex process. more...

Organism:

Theileria parva; Theileria parva strain Muguga

Type:

Expression profiling by array

Platform:

GPL7828

3 Samples

Download data: TXT

(Submitter supplied) 1plex 385k expression array representing all available RefSeq RNA sequences plus additional EST sequences for Bos taurus and including all predicted coding sequences of the bovine intracellular parasite Theileria annulata. 60 mer probe array, five probes per gene, replicated in 2 blocks. Protocol: see manufacturer's website

Organism:

Theileria annulata; Bos taurus

1 Series

20 Samples

Download data: NDF

(Submitter supplied) Custom B. bovis microarray slides were manufactured using a proprietary non-contact industrial inkjet printing process, in which oligo monomers were deposited uniformly onto specially-prepared glass slides. This in situ synthesis process printed 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. The precise inkjet process enabled the delivery of extremely small, accurate volumes (picoliters) of the chemicals to be spotted. more...

Organism:

Babesia bovis

1 Series

6 Samples

Download data: TXT

(Submitter supplied) 385K expression array representing all available RefSeq RNA sequences plus additional EST sequences for Bos taurus and including all predicted coding sequences of the bovine intracellular parasite Theileria annulata. 60 mer probe array, five probes per gene, replicated in 2 blocks. native array description file: 080829_B_taurus_JK_EXP.ndf native array description file: 080829_B_taurus_JK_EXP.ngd Protocol: Expression array - 1plex 385k expression array

Organism:

Theileria annulata; Bos taurus

1 Series

18 Samples

Download data: NDF, NGD

(Submitter supplied) 385K expression array representing all available RefSeq RNA sequences plus additional EST sequences for Bos taurus and including all predicted coding sequences of the bovine intracellular parasite Theileria annulata. 60 mer probe array, five probes per gene, replicated in 2 blocks. 080829_B_taurus_JK_EXP.ndf 080829_B_taurus_JK_EXP.ngd Protocol: Expression array - 1plex 385k expression array

Organism:

Theileria annulata; Bos taurus

1 Series

18 Samples

Download data: NDF, NGD

(Submitter supplied) Microarray design and printing was performed by Dr. Simon Kang’a at The Institute for Genomic Research (TIGR), now the J. Craig Venter Institute (JCVI). A total of 4060 unique 70 mer oligonucleotides were designed from an initial 4082 predicted ORF of the Theileria parva (Muguga) genome using the Pick70 program of ArrayOligoSelector software (http://arrayoligosel.sourceforge.net). Oligonucleotide design considerations included uniqueness, avoidance of internal self-annealing structures, narrow annealing temperature (Tm) range over the entire oligonucleotide set, masking of low-complexity regions, balanced base composition (28-30%GC) and proximity to the 3' end. more...

Organism:

Theileria parva strain Muguga

1 Series

3 Samples

Download data