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| Status |
Public on Dec 20, 2022 |
| Title |
Comparison of high throughput RNA sequences between Babesia bigemina and Babesia bovis revealed consistent differential gene expression that is required for Babesia life cycle in the vertebrate and invertebrate hosts. |
| Organism |
Babesia bigemina |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites
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| |
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| Overall design |
Parasite mRNA isolated from 3 biological replicates for each of bovine blood stage and tick kinete stage parasites were compared by RNAseq methodology using Illumina technology.
|
| Web link |
https://www.frontiersin.org/articles/10.3389/fcimb.2022.1093338/full
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| |
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| Contributor(s) |
Capelli-Peixoto J, Saelao P, Johnson WC, Kappmeyer LS, Ueti MW |
| Citation(s) |
36601308 |
| |
| Submission date |
Sep 24, 2022 |
| Last update date |
Jan 13, 2023 |
| Contact name |
Massaro Wilson Ueti |
| E-mail(s) |
massaro.utei@usda.gov
|
| Phone |
509-335-6059
|
| Organization name |
USDA ARS Animal Disease Research Unit
|
| Street address |
3003 ADBF Washington State Univ, 3003 ADBF, Grimes Way
|
| City |
Pullman |
| State/province |
Washington |
| ZIP/Postal code |
99164-6630 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL32692 |
Illumina HiSeq 2500 (Babesia bigemina) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA883971 |
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