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| Status |
Public on Apr 10, 2015 |
| Title |
Morphological and molecular descriptors of the developmental cycle of Babesia divergens parasites in human erythrocytes |
| Platform organism |
Babesia bovis |
| Sample organism |
Babesia divergens |
| Experiment type |
Expression profiling by array
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| Summary |
Human babesiosis, especially caused by the cattle derived Babesia divergens parasite, is on the increase, resulting in renewed attentiveness to this potentially life threatening emerging zoonotic disease. The molecular mechanisms underlying the pathophysiology and intra-erythrocytic development of these parasites are poorly understood. This impedes concerted efforts aimed at the discovery of novel anti-babesiacidal agents. By applying sensitive cell biological and molecular functional genomics tools, we describe the intra-erythrocytic development cycle of B. divergens parasites from immature, mono-nucleated ring forms to bi-nucleated paired piriforms and ultimately multi-nucleated tetrads that characterizes zoonotic Babesia spp. This is further correlated for the first time to nuclear content increases during intra-erythrocytic development progression, providing insight into the part of the life cycle that occurs during human infection. High-content temporal evaluation elucidated the contribution of the different stages to life cycle progression. Moreover, molecular descriptors indicate that B. divergens parasites employ physiological adaptation to in vitro cultivation. Additionally, differential expression is observed as the parasite equilibrates its developmental stages during its life cycle. Together, this information provides the first temporal evaluation of the functional transcriptome of B. divergens parasites; information that could be useful in identifying biological processes essential to parasite survival for future anti-babesiacidal discoveries.
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| Overall design |
Two-condition experiment, Untreated vs.Treated B. divergens parasites, cultured in human erythrocytes. Treatment with a piperidinyl-benzimidizalone analogue. Biological replicates: 3 untreated (control) replicates, 3 treated replicates.
The 6-sample dataset represents untreated(control) vs pooled_reference samples at various timepoints.
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| Contributor(s) |
Birkholtz L, Maritz-Olivier C, Rossouw I, Niemand J, Smit A, Olivier N, van Biljon R |
| Citation missing |
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| Submission date |
Apr 08, 2015 |
| Last update date |
Apr 10, 2015 |
| Contact name |
Ingrid Rossouw |
| E-mail(s) |
s24075681@tuks.co.za
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| Organization name |
University of Pretoria
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| Department |
Genetics
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| Street address |
Lynnwoodroad
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| City |
Pretoria |
| State/province |
Gauteng |
| ZIP/Postal code |
0002 |
| Country |
South Africa |
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| Platforms (1) |
| GPL20008 |
Agilent-036891 Babesia bovis_UP array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA280620 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67677_RAW.tar |
76.8 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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