|
| Status |
Public on Jun 08, 2016 |
| Title |
Donor1 Naïve |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Naïve CD4+ T cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Naïve CD4+ T cells
activation: naïve
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trisure following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Sample RNA was mixed with spike-in control (Agilent, # 5188-5279) A and labelled with Cy3-CTP. Reference RNA (Stratagene Universal Human Reference RNA, Agilent) was mixed with spike-in control B and labelled with Cy5-CTP.
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Stratagene Universal Human Reference RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trisure following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Sample RNA was mixed with spike-in control (Agilent, # 5188-5279) A and labelled with Cy3-CTP. Reference RNA (Stratagene Universal Human Reference RNA, Agilent) was mixed with spike-in control B and labelled with Cy5-CTP.
|
| |
|
| |
| Hybridization protocol |
Sample cRNA was mixed with reference cRNA and hybridised to SurePrint G3 Human Gene Expression 8x60K microarrays . Hybridisation and washing of the array slides was done following the specifications in the Agilent Low Input Quick Amp Labeling protocol.
|
| Scan protocol |
Arrays were scanned on an Agilent High Resolution C scanner and images were quantified using Agilent Feature Extraction Software (version 10.7)
|
| Data processing |
Fluorescence intensities were converted into log base 10 expression ratio of Cy5 to Cy3 using Feature Extraction software
Data was converted into log base 2 expression ratios of Cy3 (sample) to Cy5 (reference) signal.
|
| |
|
| Submission date |
Oct 18, 2014 |
| Last update date |
Jun 08, 2016 |
| Contact name |
Richard Jenner |
| E-mail(s) |
r.jenner@ucl.ac.uk
|
| Organization name |
UCL Cancer Institute
|
| Department |
Cancer Biology
|
| Lab |
Regulatory Genomics
|
| Street address |
72 Huntley Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13607 |
| Series (2) |
| GSE62484 |
T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation (Agilent 1) |
| GSE62486 |
T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation |
|
