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Series GSE62484 Query DataSets for GSE62484
Status Public on Jun 08, 2016
Title T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation (Agilent 1)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The transcription factor T-bet induces differentiation of CD4+ T cells into the Th1 lineage and also allows for a degree of functional plasticity. Here, we show that T-bet acts through super-enhancers to recruit the elongation factor P-TEFb. Th1-specific genes are poised for activation in Th2 cells and P-TEFb recruitment activates transcriptional elongation. T-bet also induces extensive P-TEFb binding at super-enhancers, where it acts to stimulate enhancer RNA transcription. P-TEFb inhibition selectively blocks activation of lineage-specific genes and reverses Th1-associated retinitis pathology. T-bet-mediated recruitment of P-TEFb to super-enhancers at otherwise poised genes provides a model for how lineage-specifying factors promote differentiation towards specific cell fates whilst maintaining a degree of functional plasticity.
Transcriptional profiling of human Th1 and Th2 cells
Overall design Two-condition experiment, unstimulated or reactivated Th1 and Th2 cells vs. universal reference. Biological replicates: 3
Contributor(s) Jenner R, Evans C, Hertweck A
Citation(s) 27292648
Submission date Oct 18, 2014
Last update date Nov 27, 2018
Contact name Richard Jenner
Organization name UCL Cancer Institute
Department Cancer Biology
Lab Regulatory Genomics
Street address 72 Huntley Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (23)
GSM1527738 Donor1 day 7 Th1
GSM1527739 Donor1 day 13 Th1 unactivated
GSM1527740 Donor1 day 13 Th1 activated
This SubSeries is part of SuperSeries:
GSE62486 T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation
BioProject PRJNA264231

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62484_RAW.tar 143.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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