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| Status |
Public on Jun 08, 2016 |
| Title |
T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation (Agilent 1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcription factor T-bet induces differentiation of CD4+ T cells into the Th1 lineage and also allows for a degree of functional plasticity. Here, we show that T-bet acts through super-enhancers to recruit the elongation factor P-TEFb. Th1-specific genes are poised for activation in Th2 cells and P-TEFb recruitment activates transcriptional elongation. T-bet also induces extensive P-TEFb binding at super-enhancers, where it acts to stimulate enhancer RNA transcription. P-TEFb inhibition selectively blocks activation of lineage-specific genes and reverses Th1-associated retinitis pathology. T-bet-mediated recruitment of P-TEFb to super-enhancers at otherwise poised genes provides a model for how lineage-specifying factors promote differentiation towards specific cell fates whilst maintaining a degree of functional plasticity.
Transcriptional profiling of human Th1 and Th2 cells
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| Overall design |
Two-condition experiment, unstimulated or reactivated Th1 and Th2 cells vs. universal reference. Biological replicates: 3
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| Contributor(s) |
Jenner R, Evans C, Hertweck A |
| Citation(s) |
27292648 |
| Submission date |
Oct 18, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Richard Jenner |
| E-mail(s) |
r.jenner@ucl.ac.uk
|
| Organization name |
UCL Cancer Institute
|
| Department |
Cancer Biology
|
| Lab |
Regulatory Genomics
|
| Street address |
72 Huntley Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (23)
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| This SubSeries is part of SuperSeries: |
| GSE62486 |
T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation |
|
| Relations |
| BioProject |
PRJNA264231 |
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