GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE89645 Query DataSets for GSE89645
Status Public on Aug 01, 2017
Title Expression data from mutant FoxP3-transduced CD4 T cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary FoxP3 is a central regulator of immunological tolerance, controlling the development and function of regulatory T (Treg) cells. To dissect the complex processes orchestrated by FoxP3, we investigated impacts of three autoimmune disease-associated missense FoxP3 mutations in mice.
As a initial approach, we retrovirally transduced naïve conventional T (Tconv) cells with WT or mutant (A384T and R397W) FoxP3 and analyzed the gene expression profiles of the transduced T cells.
Overall design Naïve CD4(+) CD25(-) CD45RB(high) T cells were sorted from BALB/cA mice and retrovirally transduced with either MIGR1, mouse WT FoxP3/MIGR1, A384T FoxP3/MIGR1, or R397W FoxP3/MIGR1 retrovirus. GFP(high) CD4(+) transduced T cells were sorted for RNA extraction and hybridization on Affymetrix microarrays. All cell populations analyzed were genereted in duplicates or triplicates.
Contributor(s) Hori S
Citation(s) 28778586
Submission date Nov 08, 2016
Last update date Feb 11, 2019
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (11)
GSM2385956 Mock-transduced T cells_1
GSM2385957 Mock-transduced T cells_2
GSM2385958 Mock-transduced T cells_3
This SubSeries is part of SuperSeries:
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells
BioProject PRJNA352790

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89645_RAW.tar 35.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap