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| Status |
Public on Aug 01, 2017 |
| Title |
Expression data from mutant FoxP3-transduced CD4 T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
FoxP3 is a central regulator of immunological tolerance, controlling the development and function of regulatory T (Treg) cells. To dissect the complex processes orchestrated by FoxP3, we investigated impacts of three autoimmune disease-associated missense FoxP3 mutations in mice.
As a initial approach, we retrovirally transduced naïve conventional T (Tconv) cells with WT or mutant (A384T and R397W) FoxP3 and analyzed the gene expression profiles of the transduced T cells.
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| Overall design |
Naïve CD4(+) CD25(-) CD45RB(high) T cells were sorted from BALB/cA mice and retrovirally transduced with either MIGR1, mouse WT FoxP3/MIGR1, A384T FoxP3/MIGR1, or R397W FoxP3/MIGR1 retrovirus. GFP(high) CD4(+) transduced T cells were sorted for RNA extraction and hybridization on Affymetrix microarrays. All cell populations analyzed were genereted in duplicates or triplicates.
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| Contributor(s) |
Hori S |
| Citation(s) |
28778586 |
| |
| Submission date |
Nov 08, 2016 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Shohei Hori |
| E-mail(s) |
shohei.hori@riken.jp
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| Phone |
+81-45-503-7069
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| Organization name |
RIKEN Center for Integrative Medical Sciences
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| Lab |
Laboratory for Immune Homeostasis
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| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
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| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE89744 |
Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells |
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| Relations |
| BioProject |
PRJNA352790 |
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