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Sample GSM2385959 Query DataSets for GSM2385959
Status Public on Aug 01, 2017
Title WT FoxP3-transduced T cells_1
Sample type RNA
Source name GFP(high) CD4(+) T cells transduced with WT FoxP3/MIGR1 retroviral vector
Organism Mus musculus
Characteristics strain: BALB/cA
tissue: lymph nodes and spleen
cell type: In vitro activated CD4(+) T cells
transduction: WT FoxP3/MIGR1 retroviral vector
Treatment protocol Sorted naïve CD4(+) T cells were stimulated with 0.5 ug/ml anti-CD3e (145-2C11) in the presence of 10 ng/ml recombinant mouse IL-2 and syngeneic T cell-depleted and irradiated (20Gy) spleen cells, and spin-infected with retroviral supernatants 24 hr later.
Growth protocol Retrovirally transduced T cells were cultured in RPMI1640 medium supplemented with 10% FBS, 10 mM HEPES, 1 mM Sodium Pyruvate, 50 uM 2-ME, PN/ST, and 10 ng/ml recombinant mouse IL-2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip One-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Naïve CD4(+) T cells were transduced with WT FoxP3/MIGR1 retroviral vector and GFP(high) CD4(+) cells were sorted on day 7.
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89645 Expression data from mutant FoxP3-transduced CD4 T cells
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.002373695
AFFX-BioB-M_at -0.16389227
AFFX-BioB-3_at -0.085165024
AFFX-BioC-5_at -0.25876236
AFFX-BioC-3_at -0.30203724
AFFX-BioDn-5_at -0.2211361
AFFX-BioDn-3_at -0.06980419
AFFX-CreX-5_at -0.14119434
AFFX-CreX-3_at -0.02551651
AFFX-DapX-5_at -0.3275609
AFFX-DapX-M_at -0.3791542
AFFX-DapX-3_at -0.18460941
AFFX-LysX-5_at -0.41955304
AFFX-LysX-M_at -0.015331268
AFFX-LysX-3_at -0.41390324
AFFX-PheX-5_at -0.13828564
AFFX-PheX-M_at -0.1257987
AFFX-PheX-3_at -0.1825757
AFFX-ThrX-5_at 0.22986317
AFFX-ThrX-M_at -0.10967684

Total number of rows: 45101

Table truncated, full table size 991 Kbytes.

Supplementary file Size Download File type/resource
GSM2385959_Foxp3_1.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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