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Sample GSM2385965 Query DataSets for GSM2385965
Status Public on Aug 01, 2017
Title R397W FoxP3-transduced T cells_1
Sample type RNA
Source name GFP(high) CD4(+) T cells transduced with R397W FoxP3/MIGR1 retroviral vector
Organism Mus musculus
Characteristics strain: BALB/cA
tissue: lymph nodes and spleen
cell type: In vitro activated CD4(+) T cells
transduction: R397W FoxP3/MIGR1 retroviral vector
Treatment protocol Sorted naïve CD4(+) T cells were stimulated with 0.5 ug/ml anti-CD3e (145-2C11) in the presence of 10 ng/ml recombinant mouse IL-2 and syngeneic T cell-depleted and irradiated (20Gy) spleen cells, and spin-infected with retroviral supernatants 24 hr later.
Growth protocol Retrovirally transduced T cells were cultured in RPMI1640 medium supplemented with 10% FBS, 10 mM HEPES, 1 mM Sodium Pyruvate, 50 uM 2-ME, PN/ST, and 10 ng/ml recombinant mouse IL-2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip One-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Naïve CD4(+) T cells were transduced with R397W FoxP3/MIGR1 retroviral vector and GFP(high) CD4(+) cells were sorted on day 7.
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89645 Expression data from mutant FoxP3-transduced CD4 T cells
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at 0.35357094
AFFX-BioB-M_at 0.21808004
AFFX-BioB-3_at 0.28320026
AFFX-BioC-5_at 0.14418411
AFFX-BioC-3_at -0.02292347
AFFX-BioDn-5_at 0.031817436
AFFX-BioDn-3_at 0.2513361
AFFX-CreX-5_at 0.01733017
AFFX-CreX-3_at 0.15915585
AFFX-DapX-5_at 0.3134799
AFFX-DapX-M_at 0.3596182
AFFX-DapX-3_at 0.69656944
AFFX-LysX-5_at 0.10221052
AFFX-LysX-M_at 0.5901332
AFFX-LysX-3_at 0.41704273
AFFX-PheX-5_at 0.5700612
AFFX-PheX-M_at 0.68832827
AFFX-PheX-3_at 0.7344351
AFFX-ThrX-5_at 0.91095114
AFFX-ThrX-M_at 0.6101408

Total number of rows: 45101

Table truncated, full table size 993 Kbytes.

Supplementary file Size Download File type/resource
GSM2385965_R397W_1.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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