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Sample GSM2385963 Query DataSets for GSM2385963
Status Public on Aug 01, 2017
Title A384T FoxP3-transduced T cells_2
Sample type RNA
Source name GFP(high) CD4(+) T cells transduced with A384T FoxP3/MIGR1 retroviral vector
Organism Mus musculus
Characteristics strain: BALB/cA
tissue: lymph nodes and spleen
cell type: In vitro activated CD4(+) T cells
transduction: A384T FoxP3/MIGR1 retroviral vector
Treatment protocol Sorted naïve CD4(+) T cells were stimulated with 0.5 ug/ml anti-CD3e (145-2C11) in the presence of 10 ng/ml recombinant mouse IL-2 and syngeneic T cell-depleted and irradiated (20Gy) spleen cells, and spin-infected with retroviral supernatants 24 hr later.
Growth protocol Retrovirally transduced T cells were cultured in RPMI1640 medium supplemented with 10% FBS, 10 mM HEPES, 1 mM Sodium Pyruvate, 50 uM 2-ME, PN/ST, and 10 ng/ml recombinant mouse IL-2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip One-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Naïve CD4(+) T cells were transduced with A384T FoxP3/MIGR1 retroviral vector and GFP(high) CD4(+) cells were sorted on day 7.
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89645 Expression data from mutant FoxP3-transduced CD4 T cells
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.40473366
AFFX-BioB-M_at -0.32031012
AFFX-BioB-3_at -0.47388887
AFFX-BioC-5_at -0.16800117
AFFX-BioC-3_at -0.39239788
AFFX-BioDn-5_at -0.4375391
AFFX-BioDn-3_at -0.27241802
AFFX-CreX-5_at -0.16150951
AFFX-CreX-3_at -0.06560612
AFFX-DapX-5_at -1.2865663
AFFX-DapX-M_at -1.0541558
AFFX-DapX-3_at -0.77005005
AFFX-LysX-5_at -0.38788152
AFFX-LysX-M_at -0.6476126
AFFX-LysX-3_at -0.7413845
AFFX-PheX-5_at -0.8401151
AFFX-PheX-M_at -0.8681073
AFFX-PheX-3_at -0.5409422
AFFX-ThrX-5_at -1.1643372
AFFX-ThrX-M_at -0.9885192

Total number of rows: 45101

Table truncated, full table size 994 Kbytes.

Supplementary file Size Download File type/resource
GSM2385963_A384T_2.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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