|
| Status |
Public on Aug 29, 2016 |
| Title |
Diagnosis of childhood bacterial and viral infection using host RNA expression [Discovery set] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genome-wide analysis of transcriptional profiles in children <17 years of age with bacterial or viral infections or with clinical features suggestive of infection.
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| Overall design |
Patients were recruited at or after presentation to hospital. Main inclusion criteria were presence of illness of sufficient severity to warrant blood tests for clinical reasons, in child aged <17 years. Blood was collected into PAX gene tubes (PreAnalytiX). Total RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labeled cRNA was hybridized to Illumina Human HT-12 v4 Beadchips (Discovery set) or Illumina Human HT-12 V3 Beadchips (Validation set). Numbers of arrays analysed were as follows: Definite Bacterial infection (n=52), Definite Viral infection (n=92), Probable Bacterial infection (n=42), Probable Viral infection (n=5), infections of uncertain bacterial or viral aetiology (n=49), Healthy controls (n=52).
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| |
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| Citation(s) |
27552617 |
| Submission date |
Sep 08, 2015 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Victoria Wright |
| E-mail(s) |
v.wright@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Paediatrics
|
| Lab |
Wright Fleming Institute
|
| Street address |
Norfolk Place
|
| City |
London |
| ZIP/Postal code |
W2 1PG |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (292)
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| This SubSeries is part of SuperSeries: |
| GSE72829 |
Diagnosis of childhood bacterial and viral infection using host RNA expression |
|
| Relations |
| BioProject |
PRJNA295138 |
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