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| Status |
Public on Feb 01, 2015 |
| Title |
Lung cell responses to ship diesel exhaust particles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human BEAS-2B bronchial epithelial cells were exposed directly at the air-liquid interphase towards exhaust gas and particles of a ship engine. The goal was to compare the responses towards different fuel combustions. The engine run either on diesel fuel (DF) or on Heavy Fuel Oil (HFO).
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| Overall design |
The lung cells were exposed 3 times to each combustion aerosol (DF or HFO). The duration of the exposure was 4h. The cells were seeded into transwell-inserts 24h before exposure. Within each exposure 3 transwell-inserts were exposed to the complete aerosol and 3 transwell-inserts were exposed to the filtered aerosol. Effects of the complete aerosol were referenced against the filtered aerosol to determine the effects of the aerosol particles.
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| Citation(s) |
26039251 |
| Submission date |
Dec 08, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Sebastian Oeder |
| E-mail(s) |
sebastian.oeder@lrz.tum.de
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| Organization name |
Technische Universität München
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| Department |
Center for Allergy and Environment (ZAUM)
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| Street address |
Biedersteinerstr. 29
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| City |
Munich |
| ZIP/Postal code |
80802 |
| Country |
Germany |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA269614 |
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