|
| Status |
Public on Feb 01, 2015 |
| Title |
DF_gasphase_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
BEAS-2B, 4h, DF, gas phase, replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human immortalized bronchial epithelial cells BEAS-2B
fuel type: Diesel Fuel
aerosol: filtered aerosol
|
| Treatment protocol |
In a fully automatic Air-liquid-interface exposure chamber cell cultures grown on porous membrane inserts, which supply the nutrient medium, are exposed directly to diluted aerosol from combustion sources (Fig. S2). The aerosol is humidified by steam injection to 85 % relative humidity and temperature is controlled to 37°C. The flow rate is 100 ml/min.
|
| Growth protocol |
Human immortalized bronchial epithelial cells BEAS-2B (ATCC® CRL-9609™; http://www.lgcstandards-atcc.org/Products/All/CRL-9609.aspx) were cultured in Bronchial Epithelial Growth Medium (BEGM, Lonza Inc., Walkersville, MD) supplemented with 100 units/ml penicillin/streptomycin and on plates that were pre-coated with 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen Type 1 and 0.01 mg/ml BSA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the AllPrep RNA/Protein Kit (Qiagen, Hilden, Germany).
|
| Label |
Cy3
|
| Label protocol |
Total RNA was Cy3-labeled according to the manufacturers protocol (Agilent, Waldbronn, Germany).
|
| |
|
| Hybridization protocol |
Hybridisation to Sure Print G3 Human Gene Expression Microarrays (8x60k) was erformed according to the manufacturers protocol (Agilent, Waldbronn, Germany).
|
| Scan protocol |
Scanning was performed according to the manufacturers protocol (Agilent, Waldbronn, Germany).
|
| Description |
Gene expression in BEAS-2B cells after 4h on-line exposure to filtered DF combustion aerosol
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters.
|
| |
|
| Submission date |
Dec 08, 2014 |
| Last update date |
Feb 01, 2015 |
| Contact name |
Sebastian Oeder |
| E-mail(s) |
sebastian.oeder@lrz.tum.de
|
| Organization name |
Technische Universität München
|
| Department |
Center for Allergy and Environment (ZAUM)
|
| Street address |
Biedersteinerstr. 29
|
| City |
Munich |
| ZIP/Postal code |
80802 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE63962 |
Lung cell responses to ship diesel exhaust particles |
|
