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Series GSE61694 Query DataSets for GSE61694
Status Public on Sep 17, 2015
Title MEF_OSK_O_KS_T20
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Next Generation Sequencing with differdent pluripotent transcript factor overexpression in MEF
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. The low efficiency of reprogramming limited the potential application of iPSCs. Here we found that knockdown LSD1 , which demethylates histone H3 Lys 4 or 9 , could increase iPSCs generation. It has been reported that LSD1 interaction with Oct4 which is the core factor of reprogramming. So we try to find out the different of overexpression Oct4 or Kllf4/Sox2 in MEF and LSD1 inhibitor.
Overall design Retrovirus-mediated different pluripotent transcript factor overexpression in MEF cells after 4 days collect mRNA profiles processing with Illumina MiSeq;
MEF, Mouse Embryonic Fibroblast
O, MEF infected with Oct4
KS,MEF infected with Klf4 and Sox2
OSK, MEF infected with Oct4 Klf4 and Sox2
T20, MEF infected with Oct4 Klf4 and Sox2 and treated with 20nm LSD1 inhibitor Tranylcypromine
Contributor(s) Sun H, Zheng H
Citation(s) 27481483
Submission date Sep 23, 2014
Last update date Oct 03, 2019
Contact name Hao Sun
Organization name Chinese Academy of Sciences
Lab Zheng's Lab
Street address 190 Kaiyuan Avenue
City Guangzhou
ZIP/Postal code 510530
Country China
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (5)
GSM1511383 MEF
GSM1511384 OSK
GSM1511385 O
BioProject PRJNA261855
SRA SRP047439

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61694_RAW.tar 2.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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