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Sample GSM1511384 Query DataSets for GSM1511384
Status Public on Sep 17, 2015
Title OSK
Sample type SRA
 
Source name Mouse embryonic fibroblast
Organism Mus musculus
Characteristics strain background: ICR
cell type: Mouse embryonic fibroblast
passages: 3-5
infected with: Oct4 Klf4 and Sox2
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
 
Data processing Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie
Transcripts Per Million were calculated using a protocol from RSEM
Genome_build: mm10
Supplementary_files_format_and_content: TPM
 
Submission date Sep 23, 2014
Last update date May 15, 2019
Contact name Hao Sun
E-mail(s) sun_hao@grmh-gdl.cn
Organization name Chinese Academy of Sciences
Lab Zheng's Lab
Street address 190 Kaiyuan Avenue
City Guangzhou
ZIP/Postal code 510530
Country China
 
Platform ID GPL16417
Series (1)
GSE61694 MEF_OSK_O_KS_T20
Relations
BioSample SAMN03076331
SRA SRX708969

Supplementary file Size Download File type/resource
GSM1511384_ips_osk.genes.txt.gz 600.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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